|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
8
|
|
pubmed:dateCreated |
2002-2-18
|
|
pubmed:abstractText |
Heterogeneous nuclear ribonucleoprotein K (hnRNP K) protein interacts with a subset of cellular RNAs. We used K protein as a bait in the yeast three-hybrid screen to identify RNAs that bind K protein in vivo. A large number of K protein-binding RNA clones were identified from a human hybrid RNA library. These sequences consisted of C-rich patches and were G-poor. Unexpectedly, several of the RNA clones were encoded by the mitochondrial genome. In a subsequent three-hybrid screen of a hybrid RNA library generated from a mouse liver mitochondrial genome, K protein bound RNA sequences encoded by different loci spanning nearly the entire mitochondrial genome. Western blot analysis of extracts from mitochondria and mitochondrial fractions showed that K protein is localized within mitoplasts. Reverse transcriptase PCR of RNA co-immunoprecipitated with K protein from lysates of isolated mitochondria showed that K protein is associated with several processed mitochondrial transcripts. In contrast, in the same assay, the polycistronic nascent mtRNA bound K protein weakly or not at all. Results of this study suggest that K protein acts within functional modules that are responsible for expression of genes in mitochondria.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Feb
|
|
pubmed:issn |
0021-9258
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
22
|
|
pubmed:volume |
277
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
6303-10
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:11741984-Animals,
pubmed-meshheading:11741984-Base Sequence,
pubmed-meshheading:11741984-Binding Sites,
pubmed-meshheading:11741984-Cloning, Molecular,
pubmed-meshheading:11741984-DNA, Mitochondrial,
pubmed-meshheading:11741984-DNA Primers,
pubmed-meshheading:11741984-Genes, Reporter,
pubmed-meshheading:11741984-Heterogeneous-Nuclear Ribonucleoprotein K,
pubmed-meshheading:11741984-Heterogeneous-Nuclear Ribonucleoproteins,
pubmed-meshheading:11741984-Humans,
pubmed-meshheading:11741984-Mice,
pubmed-meshheading:11741984-Mitochondria, Liver,
pubmed-meshheading:11741984-Molecular Sequence Data,
pubmed-meshheading:11741984-Polymerase Chain Reaction,
pubmed-meshheading:11741984-RNA, Heterogeneous Nuclear,
pubmed-meshheading:11741984-RNA-Binding Proteins,
pubmed-meshheading:11741984-Rabbits,
pubmed-meshheading:11741984-Ribonucleoproteins,
pubmed-meshheading:11741984-Saccharomyces cerevisiae,
pubmed-meshheading:11741984-Transcription, Genetic,
pubmed-meshheading:11741984-beta-Galactosidase
|
|
pubmed:year |
2002
|
|
pubmed:articleTitle |
Heterogeneous nuclear ribonucleoprotein K protein associates with multiple mitochondrial transcripts within the organelle.
|
|
pubmed:affiliation |
Department of Gastroenterology, Medical Center for Postgraduate Education, Maria Sklodowska-Curie Memorial Cancer Center, and the Institute of Oncology, 02-781 Warsaw, Poland. jostrow@warman.com.pl
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
