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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
2002-2-18
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pubmed:abstractText |
Brain-derived neurotrophic factor (BDNF) has been reported to exert an acute potentiation of synaptic activity. Here we examined the action of BDNF on synchronous spontaneous Ca(2+) oscillations in cultured cerebral cortical neurons prepared from postnatal 2-3-day-old rats. The synchronous spontaneous Ca(2+) oscillations began at approximately DIV 5. It was revealed that voltage-dependent Ca(2+) channels and ionotropic glutamate receptors were involved in the synchronous spontaneous oscillatory activity. BDNF potentiated the frequency of these oscillations. The BDNF-potentiated activity reached 207 +/- 20.1% of basal oscillatory activity. NT-3 and NT-4/5 also induced the potentiation. However, nerve growth factor did not. We examined the correlation between BDNF-induced glutamate release and the BDNF-potentiated oscillatory activity. Both up-regulation of phospholipase C-gamma (PLC-gamma) expression and the BDNF-induced glutamate release occurred at approximately DIV 5 when the BDNF-potentiated oscillations appeared. We confirmed that the BDNF-induced glutamate release occurred through a glutamate transporter that was dependent on the PLC-gamma/IP(3)/Ca(2+) pathway. Transporter inhibitors blocked the BDNF-potentiated oscillations, demonstrating that BDNF enhanced the glutamatergic transmissions in the developing cortical network by inducing glutamate release via a glutamate transporter.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acid Transport System X-AG,
http://linkedlifedata.com/resource/pubmed/chemical/Brain-Derived Neurotrophic Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Glutamic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Neurotransmitter Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipase C gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Type C Phospholipases,
http://linkedlifedata.com/resource/pubmed/chemical/oncogene protein trk
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
22
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pubmed:volume |
277
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6520-9
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:11741947-Amino Acid Transport System X-AG,
pubmed-meshheading:11741947-Animals,
pubmed-meshheading:11741947-Animals, Newborn,
pubmed-meshheading:11741947-Brain-Derived Neurotrophic Factor,
pubmed-meshheading:11741947-Calcium Signaling,
pubmed-meshheading:11741947-Cell Division,
pubmed-meshheading:11741947-Cells, Cultured,
pubmed-meshheading:11741947-Cerebral Cortex,
pubmed-meshheading:11741947-Glutamic Acid,
pubmed-meshheading:11741947-Isoenzymes,
pubmed-meshheading:11741947-Kinetics,
pubmed-meshheading:11741947-Mitogen-Activated Protein Kinases,
pubmed-meshheading:11741947-Neurons,
pubmed-meshheading:11741947-Neurotransmitter Agents,
pubmed-meshheading:11741947-Oncogene Proteins,
pubmed-meshheading:11741947-Oscillometry,
pubmed-meshheading:11741947-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:11741947-Phospholipase C gamma,
pubmed-meshheading:11741947-Rats,
pubmed-meshheading:11741947-Type C Phospholipases
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pubmed:year |
2002
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pubmed:articleTitle |
Brain-derived neurotrophic factor-induced potentiation of Ca(2+) oscillations in developing cortical neurons.
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pubmed:affiliation |
Division of Protein Biosynthesis, Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, Japan. numakawa@protein.osaka-u.ac.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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