Source:http://linkedlifedata.com/resource/pubmed/id/11741893
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
|
| pubmed:dateCreated |
2002-2-18
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| pubmed:databankReference | |
| pubmed:abstractText |
We have cloned a novel human GCK family kinase that has been designated as MASK (Mst3 and SOK1-related kinase). MASK is widely expressed and encodes a protein of 416 amino acid residues, with an N-terminal kinase domain and a unique C-terminal region. Like other GCK-III subfamily kinases, MASK does not activate any mitogen-activated protein kinase pathways. Wild type MASK, but not a form lacking the C terminus, exhibits homophilic binding in the yeast two-hybrid system and in coimmunoprecipitation experiments. Additionally, deletion of this C-terminal region of MASK leads to an increased kinase activity toward itself as well as toward an exogenous substrate, myelin basic protein. A potential caspase 3 cleavage site (DESDS) is present in the C-terminal region of MASK, and we show that MASK is cleaved in vitro by caspase 3. Finally, wild type and C-terminally truncated forms of MASK can both induce apoptosis upon overexpression in mammalian cells that is abrogated by CrmA, suggesting involvement of MASK in the apoptotic machinery in mammalian cells.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/MST4 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/germinal center kinases
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| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
0021-9258
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
22
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| pubmed:volume |
277
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
5929-39
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| pubmed:dateRevised |
2009-11-19
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| pubmed:meshHeading |
pubmed-meshheading:11741893-Amino Acid Motifs,
pubmed-meshheading:11741893-Amino Acid Sequence,
pubmed-meshheading:11741893-Animals,
pubmed-meshheading:11741893-Apoptosis,
pubmed-meshheading:11741893-Base Sequence,
pubmed-meshheading:11741893-Cloning, Molecular,
pubmed-meshheading:11741893-DNA Fragmentation,
pubmed-meshheading:11741893-DNA Primers,
pubmed-meshheading:11741893-Exons,
pubmed-meshheading:11741893-Germinal Center,
pubmed-meshheading:11741893-Humans,
pubmed-meshheading:11741893-Introns,
pubmed-meshheading:11741893-Mammals,
pubmed-meshheading:11741893-Molecular Sequence Data,
pubmed-meshheading:11741893-Peptide Fragments,
pubmed-meshheading:11741893-Protein Kinases,
pubmed-meshheading:11741893-Protein-Serine-Threonine Kinases,
pubmed-meshheading:11741893-Saccharomyces cerevisiae
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| pubmed:year |
2002
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| pubmed:articleTitle |
Cloning of MASK, a novel member of the mammalian germinal center kinase III subfamily, with apoptosis-inducing properties.
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| pubmed:affiliation |
Kusumi Membrane Organizer Project, Exploratory Research for Advanced Technology, Japan Science and Technology Corporation, 5-11-33 Chiyoda, Nagoya 460-0012, Japan.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|