Source:http://linkedlifedata.com/resource/pubmed/id/11739555
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
2001-12-12
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pubmed:abstractText |
CD26 is a lymphocyte marker that can anchor adenosine deaminase (ADA) on the T cell surface. We found that ADA is regulated by cytokines on the cell surface during T cell activation. By means of flow cytometry, immunofluorescence, and immunoblotting techniques, we found that interleukin (IL)-2 and IL-12 up-regulate ecto-ADA and CD26 expression. In clear contrast, IL-4 led to down-regulation of lymphocyte surface ADA without modifying the level of CD26. Moreover, neither circulating ADA transcription nor mRNA translation was regulated by cytokines. These results, along with absence of total-ADA modulation, the variable amount of ADA found in purified plasma membranes, and the different effect of Brefeldin A on the surface presence of ADA and CD26 indicated that cytokines regulate the translocation of ADA towards the cell surface through a mechanism not involving CD26. Ecto-ADA protected activated lymphocytes from the toxic effects of extracellular adenosine. Therefore, this cell surface ADA control might constitute part of the fine immunoregulatory mechanism of adenosine-mediated signaling through purinergic receptors in leukocytes.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0741-5400
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
70
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
920-30
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:11739555-Adenosine Deaminase,
pubmed-meshheading:11739555-Cell Membrane,
pubmed-meshheading:11739555-Cells, Cultured,
pubmed-meshheading:11739555-Cytokines,
pubmed-meshheading:11739555-Dipeptidyl Peptidase 4,
pubmed-meshheading:11739555-Humans,
pubmed-meshheading:11739555-Lymphocyte Activation,
pubmed-meshheading:11739555-T-Lymphocytes,
pubmed-meshheading:11739555-Up-Regulation
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pubmed:year |
2001
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pubmed:articleTitle |
Cytokines regulate membrane adenosine deaminase on human activated lymphocytes.
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pubmed:affiliation |
Department of Biochemistry, University of Santiago de Compostela, 15706 Santiago de Compostela, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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