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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
2001-12-12
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pubmed:abstractText |
In this study, we characterized the differential receptor-binding specificity, affinity, and Janus kinase-STAT activation of cellular IL-10 (cIL-10) compared with viral IL-10 (vIL-10). Only cells expressing IL-10R1 bind human IL-10 or vIL-10. IL-10R2 does not bind to cIL-10 or vIL-10 alone and its presence does not enhance the receptor-binding affinity of cIL-10 or vIL-10, but it is essential for both cIL-10- and vIL-10-mediated signal transduction and immune regulation. Responses initiated by cIL-10 and vIL-10 were compared in B cell and mast cell lines, and demonstrated that the inability of vIL-10 to stimulate immune responses, as compared with human IL-10, is due to failure to initiate signaling. Absent signal transduction is due to low level expression of cell surface IL-10R1, since overexpressing IL-10R1 allows vIL-10 to initiate cIL-10-like signals and subsequent biological responses. These results are similar in primary cells, since splenocytes respond to both cIL-10 and vIL-10, while thymocytes respond only to cIL-10 and have very low mouse IL-10R1 but not mouse IL-10R2 expression. These data demonstrate that IL-10R1 expression plays a critical role in determining whether cells respond to IL-10. Modulation of cell surface IL-10R1 density might be an important mechanism for determining whether IL-10 leads to immunostimulation or immunosuppression in vivo.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/BCRF1 protein, Human herpesvirus 4,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-10,
http://linkedlifedata.com/resource/pubmed/chemical/Milk Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Isoforms,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-10,
http://linkedlifedata.com/resource/pubmed/chemical/STAT1 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/STAT1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/STAT3 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/STAT3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/STAT5 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Stat1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Stat3 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
167
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6884-92
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:11739506-Animals,
pubmed-meshheading:11739506-B-Lymphocytes,
pubmed-meshheading:11739506-CHO Cells,
pubmed-meshheading:11739506-Cell Line,
pubmed-meshheading:11739506-Cells, Cultured,
pubmed-meshheading:11739506-Cricetinae,
pubmed-meshheading:11739506-DNA-Binding Proteins,
pubmed-meshheading:11739506-Humans,
pubmed-meshheading:11739506-Interleukin-10,
pubmed-meshheading:11739506-Lymphocyte Activation,
pubmed-meshheading:11739506-Mast Cells,
pubmed-meshheading:11739506-Mice,
pubmed-meshheading:11739506-Milk Proteins,
pubmed-meshheading:11739506-Protein Isoforms,
pubmed-meshheading:11739506-RNA, Messenger,
pubmed-meshheading:11739506-Receptors, Interleukin,
pubmed-meshheading:11739506-Receptors, Interleukin-10,
pubmed-meshheading:11739506-STAT1 Transcription Factor,
pubmed-meshheading:11739506-STAT3 Transcription Factor,
pubmed-meshheading:11739506-STAT5 Transcription Factor,
pubmed-meshheading:11739506-Signal Transduction,
pubmed-meshheading:11739506-T-Lymphocytes,
pubmed-meshheading:11739506-Trans-Activators,
pubmed-meshheading:11739506-Transcription, Genetic,
pubmed-meshheading:11739506-Viral Proteins
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pubmed:year |
2001
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pubmed:articleTitle |
Differential IL-10R1 expression plays a critical role in IL-10-mediated immune regulation.
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pubmed:affiliation |
Institute for Gene Therapy and Molecular Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA. yaozhong.ding@mssm.edu
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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