Linked Life Data

11739485

Source:http://linkedlifedata.com/resource/pubmed/id/11739485

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2001-12-12
pubmed:abstractText
The involvement of OX40 (CD134) in the activation of CD8(+) intestinal intraepithelial lymphocytes (IELs) has been studied using freshly isolated IELs and in vitro CD3-stimulated IELs. Although freshly isolated CD8(+) IELs exhibited properties of activated T cells (CD69 expression and ex vivo cytotoxicity), virtually all CD8(+) IELs from normal mice were devoid of other activation-associated properties, including a lack of expression of OX40 and the ligand for OX40 (OX40L) and an absence of intracellular IFN-gamma staining. However, OX40 and OX40L expression were rapidly up-regulated on CD8 IELs following CD3 stimulation, indicating that both markers on IELs reflect activation-dependent events. Unlike IELs, activated lymph node T cells did not express OX40L, thus indicating that OX40-OX40L communication in the intestinal epithelium is part of a novel CD8 network. Functionally, OX40 expression was exclusively associated with IELs with active intracellular IFN-gamma synthesis and markedly enhanced cell-mediated cytotoxicity. However, OX40 costimulation during CD3-mediated activation significantly suppressed IL-10 synthesis by IELs, whereas blockade of OX40-OX40L by anti-OX40L mAb markedly increased IL-10 production. These findings indicate that: 1) resident CD69(+)OX40(-) IELs constitute a population of partially activated T cells poised for rapid delivery of effector activity, 2) OX40 and OX40L expression defines IELs that have undergone recent immune activation, 3) OX40(+) IELs are significantly more efficient CTL than are OX40(-) IELs, and 4) the local OX40/OX40L system plays a critical role in regulating the magnitude of cytokine responses in the gut epithelium.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
167
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6717-23
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11739485-Animals, pubmed-meshheading:11739485-Antigens, CD27, pubmed-meshheading:11739485-Antigens, CD3, pubmed-meshheading:11739485-Cells, Cultured, pubmed-meshheading:11739485-Cytokines, pubmed-meshheading:11739485-Cytotoxicity, Immunologic, pubmed-meshheading:11739485-Female, pubmed-meshheading:11739485-Immunity, Mucosal, pubmed-meshheading:11739485-Immunophenotyping, pubmed-meshheading:11739485-Interferon-gamma, pubmed-meshheading:11739485-Interleukin-10, pubmed-meshheading:11739485-Intestinal Mucosa, pubmed-meshheading:11739485-Lymphocyte Activation, pubmed-meshheading:11739485-Membrane Glycoproteins, pubmed-meshheading:11739485-Mice, pubmed-meshheading:11739485-Mice, Inbred BALB C, pubmed-meshheading:11739485-Mice, Inbred C57BL, pubmed-meshheading:11739485-Models, Immunological, pubmed-meshheading:11739485-Receptors, OX40, pubmed-meshheading:11739485-Receptors, Tumor Necrosis Factor, pubmed-meshheading:11739485-T-Lymphocyte Subsets, pubmed-meshheading:11739485-T-Lymphocytes, Cytotoxic, pubmed-meshheading:11739485-Tumor Necrosis Factors
pubmed:year
2001
pubmed:articleTitle
Multiple levels of activation of murine CD8(+) intraepithelial lymphocytes defined by OX40 (CD134) expression: effects on cell-mediated cytotoxicity, IFN-gamma, and IL-10 regulation.
pubmed:affiliation
Department of Basic Sciences, Dental Branch, University of Texas Health Science Center, Houston, TX 77030, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.