The immunosuppressive activity of mycophenolate mofetil (MMF) is based on the reversible inhibition of inosine 5'-monophosphate dehydrogenase (IMPDH) by mycophenolic acid (MPA). It was the aim of this study to develop a nonradioactive method for specific measurement of IMPDH activity in isolated peripheral mononuclear cells (MNC).
Department of Internal Medicine, Nephrology, University Medical Center Charité, Campus Mitte, Humboldt University, Schumannstr. 20/21, 10098 Berlin, Germany. petra.glander@charite.de