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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2001-12-12
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pubmed:abstractText |
Background/Aims: Hepatocellular availability of S-adenosylmethionine, the principal biological methyl donor, is compromised in situations of liver damage. S-Adenosylmethionine administration alleviates experimental liver injury and increases survival in cirrhotic patients. The mechanisms behind these beneficial effects of S-adenosylmethionine are not completely known. An inflammatory component is common to many of the pathological conditions in which S-adenosylmethionine grants protection to the liver. This notion led us to study the effect of S-adenosylmethionine administration on hepatic nitric oxide synthase-2 induction in response to bacterial lipopolysaccharide and proinflammatory cytokines. Methods: The effect of S-adenosylmethionine on nitric oxide synthase-2 expression was assessed in rats challenged with bacterial lipopolysaccharide and in isolated rat hepatocytes treated with proinflammatory cytokines. Interactions between S-adenosylmethionine and cytokines on nuclear factor kappa B activation and nitric oxide synthase-2 promoter transactivation were studied in isolated rat hepatocytes and HepG2 cells, respectively. Results: S-Adenosylmethionine attenuated the induction of nitric oxide synthase-2 in the liver of lipopolysaccharide-treated rats and in cytokine-treated hepatocytes. S-Adenosylmethionine accelerated the resynthesis of inhibitor kappa B alpha, blunted the activation of nuclear factor kappa B and reduced the transactivation of nitric oxide synthase-2 promoter. Conclusions: Our findings indicate that the hepatoprotective actions of S-adenosylmethionine may be mediated in part through the modulation of nitric oxide production.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Drug Combinations,
http://linkedlifedata.com/resource/pubmed/chemical/I kappa B beta protein,
http://linkedlifedata.com/resource/pubmed/chemical/I-kappa B Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Inflammation Mediators,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappaB inhibitor alpha,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type II,
http://linkedlifedata.com/resource/pubmed/chemical/Nos2 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/S-Adenosylmethionine
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0168-8278
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
35
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
692-9
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pubmed:dateRevised |
2010-3-23
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pubmed:meshHeading |
pubmed-meshheading:11738094-Animals,
pubmed-meshheading:11738094-Cells, Cultured,
pubmed-meshheading:11738094-Cytokines,
pubmed-meshheading:11738094-DNA-Binding Proteins,
pubmed-meshheading:11738094-Drug Combinations,
pubmed-meshheading:11738094-Gene Expression Regulation,
pubmed-meshheading:11738094-Hepatocytes,
pubmed-meshheading:11738094-I-kappa B Proteins,
pubmed-meshheading:11738094-Inflammation Mediators,
pubmed-meshheading:11738094-Lipopolysaccharides,
pubmed-meshheading:11738094-Liver,
pubmed-meshheading:11738094-Male,
pubmed-meshheading:11738094-NF-kappa B,
pubmed-meshheading:11738094-Nitric Oxide,
pubmed-meshheading:11738094-Nitric Oxide Synthase,
pubmed-meshheading:11738094-Nitric Oxide Synthase Type II,
pubmed-meshheading:11738094-Promoter Regions, Genetic,
pubmed-meshheading:11738094-Rats,
pubmed-meshheading:11738094-Rats, Wistar,
pubmed-meshheading:11738094-S-Adenosylmethionine
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pubmed:year |
2001
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pubmed:articleTitle |
S-Adenosylmethionine modulates inducible nitric oxide synthase gene expression in rat liver and isolated hepatocytes.
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pubmed:affiliation |
Unidad de Hepatología, Hospital Universitario de la Princesa, Universidad Autónoma, Madrid, Spain
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pubmed:publicationType |
Journal Article
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