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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
25
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pubmed:dateCreated |
2001-12-5
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pubmed:abstractText |
Iron homeostasis in the yeast Saccharomyces cerevisiae is regulated at the transcriptional level by Aft1p, which activates the expression of its target genes in response to low-iron conditions. The yeast genome contains a paralog of AFT1, which has been designated AFT2. To establish whether AFT1 and AFT2 have overlapping functions, a mutant containing a double aft1Deltaaft2Delta deletion was generated. Growth assays established that the single aft2Delta strain exhibited no iron-dependent phenotype. However, the double-mutant aft1Deltaaft2Delta strain was more sensitive to low-iron growth conditions than the single-mutant aft1Delta strain. A mutant allele of AFT2 (AFT2-1(up)), or overexpression of the wild-type AFT2 gene, led to partial complementation of the respiratory-deficient phenotype of the aft1Delta strain. The AFT2-1(up) allele also increased the uptake of (59)Fe in an aft1Delta strain. DNA microarrays were used to identify genes regulated by AFT2. Some of the AFT2-regulated genes are known to be regulated by Aft1p; however, AFT2-1(up)-dependent activation was independent of Aft1p. The kinetics of induction of two genes activated by the AFT2-1(up) allele are consistent with Aft2p acting as a direct transcriptional factor. Truncated forms of Aft1p and Aft2p bound to a DNA duplex containing the Aft1p binding site in vitro. The wild-type allele of AFT2 activated transcription in response to growth under low-iron conditions. Together, these data suggest that yeast has a second regulatory pathway for the iron regulon, with AFT1 and AFT2 playing partially redundant roles.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11734641-10571001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11734641-10588895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11734641-10608875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11734641-10744769,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11734641-10747942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11734641-10884426,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11734641-11027285,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11734641-11102521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11734641-11112771,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11734641-11390404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11734641-11448968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11734641-1400393,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11734641-2021081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11734641-2659436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11734641-7622457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11734641-7720713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11734641-8657150,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11734641-8670839,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11734641-9192896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11734641-9200812,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11734641-9381177,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11734641-9516467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11734641-9584169,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11734641-9736617
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Aft2 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Fungal,
http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Iron,
http://linkedlifedata.com/resource/pubmed/chemical/RCS1 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
4
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pubmed:volume |
98
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
14322-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:11734641-Alleles,
pubmed-meshheading:11734641-Amino Acid Sequence,
pubmed-meshheading:11734641-Base Sequence,
pubmed-meshheading:11734641-Binding Sites,
pubmed-meshheading:11734641-DNA, Fungal,
pubmed-meshheading:11734641-Fungal Proteins,
pubmed-meshheading:11734641-Gene Deletion,
pubmed-meshheading:11734641-Gene Expression,
pubmed-meshheading:11734641-Genes, Fungal,
pubmed-meshheading:11734641-Homeostasis,
pubmed-meshheading:11734641-Iron,
pubmed-meshheading:11734641-Molecular Sequence Data,
pubmed-meshheading:11734641-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:11734641-Phenotype,
pubmed-meshheading:11734641-Promoter Regions, Genetic,
pubmed-meshheading:11734641-Protein Binding,
pubmed-meshheading:11734641-Saccharomyces cerevisiae,
pubmed-meshheading:11734641-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:11734641-Sequence Homology, Amino Acid,
pubmed-meshheading:11734641-Trans-Activators,
pubmed-meshheading:11734641-Transcription Factors
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pubmed:year |
2001
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pubmed:articleTitle |
A second iron-regulatory system in yeast independent of Aft1p.
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pubmed:affiliation |
University of Utah Health Sciences Center, Department of Medicine, Salt Lake City, Utah 84132, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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