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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2001-12-4
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pubmed:databankReference |
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pubmed:abstractText |
Several classes of cytoplasmic proteins have been found to interact specifically with the carboxyl-terminal cytoplasmic region of the angiotensin II type 1 (AT(1)) receptor to regulate different aspects of AT(1) receptor physiology. The murine Angiotensin II Receptor-Associated Protein (Agtrap) is a new member of them. We have recently cloned a new human gene cDNA that codes for a homolog of the murine Agtrap protein from a human fetal brain cDNA library. The deduced polypeptide product of the cDNA is 22 kDa in size, and its DNA and amino acid sequences are 85 and 77% identical to those of the mouse Agtrap gene, respectively. Hence we have named it the human Angiotensin II Receptor-Associated Protein (AGTRAP) gene. The mRNA of AGTRAP was most abundantly expressed in kidney, heart, pancreas and thyroid. Using the yeast two-hybrid screening of a human fetal brain cDNA library, we have identified a new interaction partner of the human AGTRAP protein, RACK1 (Receptor of Activated Protein C Kinase). The AGTRAP-RACK1 interaction was confirmed by GST fusion protein pull-down assays, co-immunoprecipitation and surface plasmon resonance. We suggest that the AGTRAP-RACK1 interaction may help to recruit signaling complex to the AT(1) receptor to affect AT(1) receptor signaling.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Angiotensin, Type 1,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Angiotensin,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/peptide I
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1357-2725
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pubmed:author |
pubmed-author:ChengChaoC,
pubmed-author:GuShaohuaS,
pubmed-author:HuangYanY,
pubmed-author:MaoYuminY,
pubmed-author:QuZ FZF,
pubmed-author:TangRongR,
pubmed-author:VighB JBJ,
pubmed-author:XuMingM,
pubmed-author:YingKangK,
pubmed-author:ZengLiL,
pubmed-author:ZhaoWeiW,
pubmed-author:ZhouZongxiangZ
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pubmed:issnType |
Print
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pubmed:volume |
34
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
93-102
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:11733189-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:11733189-Amino Acid Sequence,
pubmed-meshheading:11733189-Animals,
pubmed-meshheading:11733189-Base Sequence,
pubmed-meshheading:11733189-Cloning, Molecular,
pubmed-meshheading:11733189-DNA, Complementary,
pubmed-meshheading:11733189-Female,
pubmed-meshheading:11733189-Humans,
pubmed-meshheading:11733189-Mice,
pubmed-meshheading:11733189-Molecular Sequence Data,
pubmed-meshheading:11733189-Peptides,
pubmed-meshheading:11733189-Pregnancy,
pubmed-meshheading:11733189-Protein Binding,
pubmed-meshheading:11733189-RNA, Messenger,
pubmed-meshheading:11733189-Receptor, Angiotensin, Type 1,
pubmed-meshheading:11733189-Receptors, Angiotensin,
pubmed-meshheading:11733189-Recombinant Proteins,
pubmed-meshheading:11733189-Sequence Homology, Amino Acid,
pubmed-meshheading:11733189-Signal Transduction,
pubmed-meshheading:11733189-Species Specificity,
pubmed-meshheading:11733189-Tissue Distribution,
pubmed-meshheading:11733189-Two-Hybrid System Techniques
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pubmed:year |
2002
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pubmed:articleTitle |
Identification and characterization of AGTRAP, a human homolog of murine Angiotensin II Receptor-Associated Protein (Agtrap).
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pubmed:affiliation |
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, People's Republic of China.
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pubmed:publicationType |
Journal Article,
In Vitro
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