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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2001-12-4
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pubmed:abstractText |
TAO1 and TAO2 are recently described protein kinases whose initial characterization has placed them at the mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) kinase kinase (MEKK) level of stress-responsive MAPK pathways. Because their physiological roles have not been identified, we sought to study their C. elegans homolog to learn more about their functions. kin-18 encodes a previously uncharacterized protein in C. elegans whose catalytic domain shares over 60% identity with TAO1 and TAO2. We demonstrate that KIN-18 is a protein of 120 kDa whose promoter is active in the pharynx and intestine of C. elegans. To learn more about TAO/KIN-18 function, we studied how expression of constitutively active forms of TAO1 or KIN-18 would affect the physiology of intact worms. Strains of C. elegans expressing active forms of TAO1 or KIN-18 exhibit altered pharyngeal electrophysiology as measured by electropharyngeogram. These worms grow more slowly and lay fewer eggs, phenotypes that could result from reduced feeding. We have also identified a C. elegans gene that encodes a protein kinase similar to mammalian MAPK/ERK Kinase (MEK) 4 whose promoter is active in the pharynx. It is phosphorylated by TAO1 in vitro and physically interacts with TAO1.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0378-1119
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
28
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pubmed:volume |
279
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
137-47
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:11733138-Amino Acid Sequence,
pubmed-meshheading:11733138-Animals,
pubmed-meshheading:11733138-Blotting, Western,
pubmed-meshheading:11733138-Caenorhabditis elegans,
pubmed-meshheading:11733138-Cell Line,
pubmed-meshheading:11733138-Feeding Behavior,
pubmed-meshheading:11733138-Gene Expression Regulation,
pubmed-meshheading:11733138-Green Fluorescent Proteins,
pubmed-meshheading:11733138-Helminth Proteins,
pubmed-meshheading:11733138-Humans,
pubmed-meshheading:11733138-Intestines,
pubmed-meshheading:11733138-Luminescent Proteins,
pubmed-meshheading:11733138-MAP Kinase Kinase Kinases,
pubmed-meshheading:11733138-Molecular Sequence Data,
pubmed-meshheading:11733138-Molecular Weight,
pubmed-meshheading:11733138-Mutagenesis, Insertional,
pubmed-meshheading:11733138-Mutation,
pubmed-meshheading:11733138-Pharynx,
pubmed-meshheading:11733138-Phenotype,
pubmed-meshheading:11733138-Promoter Regions, Genetic,
pubmed-meshheading:11733138-Protein Kinases,
pubmed-meshheading:11733138-Recombinant Fusion Proteins,
pubmed-meshheading:11733138-Sequence Alignment,
pubmed-meshheading:11733138-Sequence Homology, Amino Acid
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pubmed:year |
2001
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pubmed:articleTitle |
kin-18, a C. elegans protein kinase involved in feeding.
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pubmed:affiliation |
Department of Pharmacology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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