Linked Life Data

11731621

Source:http://linkedlifedata.com/resource/pubmed/id/11731621

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2001-12-3
pubmed:abstractText
Peptide hormones and cAMP acutely stimulate steroid biosynthesis by accelerating the transport of cholesterol into the mitochondria. The peripheral-type benzodiazepine receptor (PBR) has been shown to be an indispensable element of the cholesterol transport machinery. Using the yeast two-hybrid system and PBR as bait, we identified a protein that interacts with PBR, the PBR-associated protein PAP7. Using the regulatory subunit RIalpha of PKA as bait, we also isolated PAP7. Glutathione-S-transferase -PAP7 interacted with both the mitochondrial PBR and cytosolic PKA-RIalpha in MA-10 Leydig cells. PAP7 is a novel 52-kDa protein present in mouse, rat, and human tissues, and it has a major 3-kb mRNA transcript in all tissues examined. Immunohistochemical and in situ hybridization studies indicated that PAP7 is highly expressed in the gonads, adrenal, hippocampus, and distinct brain neuronal and glial populations. Overexpression of the full length PAP7 increased the hCG-induced steroid production. However, overexpression of a partial PAP7, which includes the PBR- and PKA-RIalpha-binding domains, inhibited the hormone-stimulated cholesterol transport and steroid synthesis. Treatment of MA-10 cells with oligonucleotides antisense to PAP7 also inhibited the hCG-stimulated steroid formation, suggesting that PAP7 is a functional element of the hormone-induced signal transduction cascade leading to steroidogenesis. PAP7 may function by targeting the PKA isoenzyme to organelles rich in PBR, i.e. mitochondria, where phosphorylation of specific protein substrates may induce the reorganization of PBR topography and function.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0888-8809
pubmed:author
pubmed:issnType
Print
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2211-28
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:11731621-Adaptor Proteins, Signal Transducing, pubmed-meshheading:11731621-Amino Acid Sequence, pubmed-meshheading:11731621-Animals, pubmed-meshheading:11731621-Base Sequence, pubmed-meshheading:11731621-Blotting, Northern, pubmed-meshheading:11731621-Blotting, Western, pubmed-meshheading:11731621-Cloning, Molecular, pubmed-meshheading:11731621-Cyclic AMP-Dependent Protein Kinases, pubmed-meshheading:11731621-Gene Expression Regulation, pubmed-meshheading:11731621-Humans, pubmed-meshheading:11731621-Immunohistochemistry, pubmed-meshheading:11731621-In Situ Hybridization, pubmed-meshheading:11731621-Leydig Cells, pubmed-meshheading:11731621-Male, pubmed-meshheading:11731621-Membrane Proteins, pubmed-meshheading:11731621-Mice, pubmed-meshheading:11731621-Molecular Sequence Data, pubmed-meshheading:11731621-Pregnenolone, pubmed-meshheading:11731621-Progesterone, pubmed-meshheading:11731621-Receptors, GABA-A, pubmed-meshheading:11731621-Sequence Analysis, DNA, pubmed-meshheading:11731621-Two-Hybrid System Techniques
pubmed:year
2001
pubmed:articleTitle
Identification, localization, and function in steroidogenesis of PAP7: a peripheral-type benzodiazepine receptor- and PKA (RIalpha)-associated protein.
pubmed:affiliation
Division of Hormone Research, Department of Cell Biology, Georgetown University School of Medicine, Washington, DC 20007, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't