Linked Life Data

11731449

Source:http://linkedlifedata.com/resource/pubmed/id/11731449

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
23
pubmed:dateCreated
2001-12-3
pubmed:abstractText
The surfaces of land plants are covered with a cuticle that is essential for retention of water. Epidermal surfaces of Arabidopsis thaliana embryos and juvenile plants that were homozygous for abnormal leaf shape1 (ale1) mutations were defective, resulting in excessive water loss and organ fusion in young plants. In ale1 embryos, the cuticle was rudimentary and remnants of the endosperm remained attached to developing embryos. Juvenile plants had a similar abnormal cuticle. The ALE1 gene was isolated using a transposon-tagged allele ale1-1. The predicted ALE1 amino acid sequence was homologous to those of subtilisin-like serine proteases. The ALE1 gene was found to be expressed within certain endosperm cells adjacent to the embryo and within the young embryo. Expression was not detected after germination. Our results suggest that the putative protease ALE1 affects the formation of cuticle on embryos and juvenile plants and that an appropriate cuticle is required for separation of the endosperm from the embryo and for prevention of organ fusion.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0950-1991
pubmed:author
pubmed:issnType
Print
pubmed:volume
128
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4681-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11731449-Alleles, pubmed-meshheading:11731449-Amino Acid Sequence, pubmed-meshheading:11731449-Arabidopsis, pubmed-meshheading:11731449-Arabidopsis Proteins, pubmed-meshheading:11731449-Base Sequence, pubmed-meshheading:11731449-DNA, Complementary, pubmed-meshheading:11731449-DNA, Plant, pubmed-meshheading:11731449-DNA Footprinting, pubmed-meshheading:11731449-Gene Expression Regulation, Developmental, pubmed-meshheading:11731449-Gene Expression Regulation, Enzymologic, pubmed-meshheading:11731449-Gene Expression Regulation, Plant, pubmed-meshheading:11731449-Genes, Plant, pubmed-meshheading:11731449-Molecular Sequence Data, pubmed-meshheading:11731449-Mutation, pubmed-meshheading:11731449-Sequence Homology, Amino Acid, pubmed-meshheading:11731449-Serine Endopeptidases, pubmed-meshheading:11731449-Subtilisins
pubmed:year
2001
pubmed:articleTitle
A subtilisin-like serine protease is required for epidermal surface formation in Arabidopsis embryos and juvenile plants.
pubmed:affiliation
Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya 464-8602, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't