Source:http://linkedlifedata.com/resource/pubmed/id/11730930
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3-4
|
| pubmed:dateCreated |
2001-12-3
|
| pubmed:databankReference | |
| pubmed:abstractText |
Mammary-associated serum amyloid A 3 (M-SAA3) was secreted at highly elevated levels in bovine, equine and ovine colostrum and found at lower levels in milk 4 days postparturition. N-terminal sequencing of the mature M-SAA3 protein from all the three species revealed a conserved four amino acid motif (TFLK) within the first eight residues. This motif has not been reported to be present in any of the hepatically-produced acute phase SAA (A-SAA) isoforms. Cloning of the bovine M-Saa3 cDNA from mammary gland epithelial cells revealed an open reading frame that encoded a precursor protein of 131 amino acids which included an 18 amino acid signal peptide. The predicted 113 residue mature M-SAA3 protein had a theoretical molecular mass of 12,826Da that corresponded with the observed 12.8kDa molecular mass obtained for M-SAA3 in immunoblot analysis. The high abundance of this extrahepatically produced SAA3 isoform in the colostrum of healthy animals suggests that M-SAA3 may play an important functional role associated with newborn adaptation to extrauterine life and possibly mammary tissue remodeling.
|
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0165-2427
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
83
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
203-11
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:11730930-Amino Acid Motifs,
pubmed-meshheading:11730930-Amino Acid Sequence,
pubmed-meshheading:11730930-Animals,
pubmed-meshheading:11730930-Base Sequence,
pubmed-meshheading:11730930-Cattle,
pubmed-meshheading:11730930-Chromatography, Affinity,
pubmed-meshheading:11730930-Colostrum,
pubmed-meshheading:11730930-DNA,
pubmed-meshheading:11730930-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:11730930-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:11730930-Horses,
pubmed-meshheading:11730930-Mammary Glands, Animal,
pubmed-meshheading:11730930-Milk,
pubmed-meshheading:11730930-Molecular Sequence Data,
pubmed-meshheading:11730930-Molecular Weight,
pubmed-meshheading:11730930-Protein Isoforms,
pubmed-meshheading:11730930-RNA,
pubmed-meshheading:11730930-Rabbits,
pubmed-meshheading:11730930-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11730930-Sequence Analysis, DNA,
pubmed-meshheading:11730930-Sequence Homology, Amino Acid,
pubmed-meshheading:11730930-Serum Amyloid A Protein,
pubmed-meshheading:11730930-Sheep
|
| pubmed:year |
2001
|
| pubmed:articleTitle |
Elevated extrahepatic expression and secretion of mammary-associated serum amyloid A 3 (M-SAA3) into colostrum.
|
| pubmed:affiliation |
Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE 68198, USA. tmcdonal@unmc.edu
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|