Linked Life Data

11726138

Source:http://linkedlifedata.com/resource/pubmed/id/11726138

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2001-11-29
pubmed:abstractText
The idiotypic determinants associated with the variable regions of antibody molecules are known to function as tumor-associated antigens (TAAs). However, there is no clear-cut evidence documenting their efficacy in inducing TAA-specific cytotoxic T-lymphocytes (CTLs). In most previous studies, idiopeptides were implicated in elicitation of TAA-specific CD4+ T-cells. Using a murine B-cell lymphoma, 2C3, we earlier demonstrated induction of splenic CD4+ and CD8+ T-lymphocytes directed to idiotypic Ig of the tumor. In the present study, we provide more direct evidence of the existence of Id-specific CTLs in the spleens of 2C3 bearing BALB/c mice using an scFv-transfectoma, P815A4, as a target. While both P815A4 and 2C3 cells were equally susceptible to cytolysis by the effector cells, lysis was evident only during early tumor progression. Moribund animals at the late stage of tumor growth failed to demonstrate any significant cytotoxic immune response against either tumor. Antibodies to MHC class I alleles Kd, Dd, Ld, beta2m and CD8 molecules all inhibited cytotoxicity. The CTL population from early tumor-bearers recognized 2C3 tumor in the context of all major H-2d alleles; however, in case of P815A4 cells, it was restricted to Kd and Dd alleles only. Based on these antibody inhibition studies, it appears that the idiopeptides generated in both tumors are in some way different, yet they were recognized equally by CTLs not only from the tumor-bearers but also by CTLs from 2C3-hyperimmune mice. It appears that scFv-containing transfectomas expressing antibody variable region epitopes would be useful for both elucidating CTL-defined idiopeptides and monitoring TAA-specific CTL response in tumor-bearing animals.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0340-7004
pubmed:author
pubmed:issnType
Print
pubmed:volume
50
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
437-44
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:11726138-Alleles, pubmed-meshheading:11726138-Animals, pubmed-meshheading:11726138-Antibodies, Monoclonal, pubmed-meshheading:11726138-Antigen Presentation, pubmed-meshheading:11726138-Antigens, CD8, pubmed-meshheading:11726138-Antigens, Neoplasm, pubmed-meshheading:11726138-CD4-Positive T-Lymphocytes, pubmed-meshheading:11726138-Genes, Immunoglobulin, pubmed-meshheading:11726138-Genes, MHC Class I, pubmed-meshheading:11726138-H-2 Antigens, pubmed-meshheading:11726138-Immunoglobulin Fragments, pubmed-meshheading:11726138-Immunoglobulin Idiotypes, pubmed-meshheading:11726138-Immunoglobulin Variable Region, pubmed-meshheading:11726138-Lymphoma, B-Cell, pubmed-meshheading:11726138-Mast-Cell Sarcoma, pubmed-meshheading:11726138-Mice, pubmed-meshheading:11726138-Mice, Inbred BALB C, pubmed-meshheading:11726138-Mice, Inbred DBA, pubmed-meshheading:11726138-Receptors, Antigen, T-Cell, alpha-beta, pubmed-meshheading:11726138-Spleen, pubmed-meshheading:11726138-T-Lymphocytes, Cytotoxic, pubmed-meshheading:11726138-Transfection, pubmed-meshheading:11726138-Tumor Cells, Cultured
pubmed:year
2001
pubmed:articleTitle
A stable single-chain variable fragment expressing transfectoma demonstrates induction of idiotype-specific cytotoxic T-cells during early growth stages of a murine B-lymphoma.
pubmed:affiliation
Department of Life Sciences, Indiana State University, Terre Haute, IN 47809, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't