Linked Life Data

11725637

Source:http://linkedlifedata.com/resource/pubmed/id/11725637

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2001-11-29
pubmed:abstractText
The dwarf gene Rht3 derived from Tom Thumb variety, a Tibetan wheat of China, is a dominant gene with the insensitivity to gibberellic acid. Rht3 shows to depress alpha-amylase activity and hence enchance the resistance to preharvest sprouting mainly through its effect on the expression of alpha-Amy1. Near isogenic lines with Rht3 and their segregating population were analyzed by PCR and RFLP techonology. In RAPD analysis, out of 310 random primers (10 bp) screened, only three primers of UBC389, OPV-06 and S1060 revealed polymorphisms in NIL from 310 random primers. Fragments S1060(1900) and S1060(2000) amplified by primer S1060 were shown to be linked to Rht3 with a genetic distance 7.1 cM and 9.2 cM. Fifty-three probes specific for short arms of homoeologous group 4 were screened in RFLP analysis. Xpsr584, XksuF8 and Xcdo38 showed polymorphisms between the NIL. The linkage analysis indicateded that Xpsr584 was linked to Rht3 with a genetic distance 8.0 cM.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0379-4172
pubmed:author
pubmed:issnType
Print
pubmed:volume
28
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1028-33
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
[Molecular markers linked to dwarf gene Rht3 in wheat].
pubmed:affiliation
Key Lab of Crop cytogenetics, ministry of Agriculture, Nanjing Agri. Univ, Nanjing 210095, China.
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't