Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2001-11-28
pubmed:abstractText
We have shown previously that Ipl1 and Sli15 are required for chromosome segregation in Saccharomyces cerevisiae. Sli15 associates directly with the Ipl1 protein kinase and these two proteins colocalize to the mitotic spindle. We show here that Sli15 stimulates the in vitro, and likely in vivo, kinase activity of Ipl1, and Sli15 facilitates the association of Ipl1 with the mitotic spindle. The Ipl1-binding and -stimulating activities of Sli15 both reside within a region containing homology to the metazoan inner centromere protein (INCENP). Ipl1 and Sli15 also bind to Dam1, a microtubule-binding protein required for mitotic spindle integrity and kinetochore function. Sli15 and Dam1 are most likely physiological targets of Ipl1 since Ipl1 can phosphorylate both proteins efficiently in vitro, and the in vivo phosphorylation of both proteins is reduced in ipl1 mutants. Some dam1 mutations exacerbate the phenotype of ipl1 and sli15 mutants, thus providing evidence that Dam1 interactions with Ipl1-Sli15 are functionally important in vivo. Similar to Dam1, Ipl1 and Sli15 each bind to microtubules directly in vitro, and they are associated with yeast centromeric DNA in vivo. Given their dual association with microtubules and kinetochores, Ipl1, Sli15, and Dam1 may play crucial roles in regulating chromosome-spindle interactions or in the movement of kinetochores along microtubules.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11724818-10072382, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724818-10377410, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724818-10385519, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724818-10397771, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724818-10511710, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724818-10523496, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724818-10975519, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724818-10996078, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724818-11050385, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724818-11149931, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724818-11179222, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724818-11266451, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724818-11266459, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724818-11511347, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724818-2659436, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724818-3047011, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724818-3316246, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724818-7565759, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724818-7903220, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724818-8007975, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724818-8120098, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724818-8242751, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724818-8293973, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724818-8368005, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724818-8408220, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724818-8978031, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724818-9230309, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724818-9407032, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724818-9490714, http://linkedlifedata.com/resource/pubmed/commentcorrection/11724818-9817759
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Chromosomal Proteins, Non-Histone, http://linkedlifedata.com/resource/pubmed/chemical/Cytoskeletal Proteins, http://linkedlifedata.com/resource/pubmed/chemical/DAM1protein, S cerevisiae, http://linkedlifedata.com/resource/pubmed/chemical/DUO1 protein, S cerevisiae, http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins, http://linkedlifedata.com/resource/pubmed/chemical/IPL1 protein, S cerevisiae, http://linkedlifedata.com/resource/pubmed/chemical/Indicators and Reagents, http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides..., http://linkedlifedata.com/resource/pubmed/chemical/Luminescent Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Microtubule-Associated Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-9525
pubmed:author
pubmed:issnType
Print
pubmed:day
26
pubmed:volume
155
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
763-74
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:11724818-Animals, pubmed-meshheading:11724818-Chromosomes, pubmed-meshheading:11724818-Phosphorylation, pubmed-meshheading:11724818-Saccharomyces cerevisiae, pubmed-meshheading:11724818-Indicators and Reagents, pubmed-meshheading:11724818-Saccharomyces cerevisiae Proteins, pubmed-meshheading:11724818-Microtubules, pubmed-meshheading:11724818-Mitotic Spindle Apparatus, pubmed-meshheading:11724818-Cell Fractionation, pubmed-meshheading:11724818-Protein Kinases, pubmed-meshheading:11724818-Chromosomal Proteins, Non-Histone, pubmed-meshheading:11724818-Cytoskeletal Proteins, pubmed-meshheading:11724818-Microtubule-Associated Proteins, pubmed-meshheading:11724818-Luminescent Proteins, pubmed-meshheading:11724818-Intracellular Signaling Peptides and Proteins, pubmed-meshheading:11724818-Two-Hybrid System Techniques, pubmed-meshheading:11724818-Protein-Serine-Threonine Kinases, pubmed-meshheading:11724818-Recombinant Fusion Proteins
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