Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2001-11-27
pubmed:abstractText
The C repeat region of the M6 protein (M6c) from Streptococcus pyogenes was expressed within the Pip bacteriophage receptor on the surface of Lactococcus lactis. M6c was also detected in the culture medium. The pip-emm6c allele was integrated into the chromosome and stably expressed without antibiotic selection. The level of cell-associated surface expression of PipM6c was 0.015% of total cellular protein. The amount of PipM6c on the cell surface was increased about 17-fold by expressing pip-emm6c from a high-copy-number plasmid. Replacing the native pip promoter with stronger promoters isolated previously from Lactobacillus acidophilus increased surface expression of PipM6c from the high-copy-number plasmid up to 27-fold. Concomitantly, the amount of PipM6c in the medium increased 113-fold. The amount of PipM6c did not vary greatly between exponential- and stationary-phase cultures. Western blots indicated that the full-length PipM6c protein and most of the numerous proteolytic products were found only on the cell surface, whereas only one proteolytic fragment was found in the culture medium.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11722881-10958782, http://linkedlifedata.com/resource/pubmed/commentcorrection/11722881-11068681, http://linkedlifedata.com/resource/pubmed/commentcorrection/11722881-1324906, http://linkedlifedata.com/resource/pubmed/commentcorrection/11722881-1342637, http://linkedlifedata.com/resource/pubmed/commentcorrection/11722881-1628895, http://linkedlifedata.com/resource/pubmed/commentcorrection/11722881-1696296, http://linkedlifedata.com/resource/pubmed/commentcorrection/11722881-1910034, http://linkedlifedata.com/resource/pubmed/commentcorrection/11722881-1917843, http://linkedlifedata.com/resource/pubmed/commentcorrection/11722881-1943817, http://linkedlifedata.com/resource/pubmed/commentcorrection/11722881-2117575, http://linkedlifedata.com/resource/pubmed/commentcorrection/11722881-2125811, http://linkedlifedata.com/resource/pubmed/commentcorrection/11722881-2428914, http://linkedlifedata.com/resource/pubmed/commentcorrection/11722881-2458320, http://linkedlifedata.com/resource/pubmed/commentcorrection/11722881-2495760, http://linkedlifedata.com/resource/pubmed/commentcorrection/11722881-2660266, http://linkedlifedata.com/resource/pubmed/commentcorrection/11722881-2668182, http://linkedlifedata.com/resource/pubmed/commentcorrection/11722881-271968, http://linkedlifedata.com/resource/pubmed/commentcorrection/11722881-2985470, http://linkedlifedata.com/resource/pubmed/commentcorrection/11722881-3043172, http://linkedlifedata.com/resource/pubmed/commentcorrection/11722881-3511046, http://linkedlifedata.com/resource/pubmed/commentcorrection/11722881-3883896, http://linkedlifedata.com/resource/pubmed/commentcorrection/11722881-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/11722881-6575708, http://linkedlifedata.com/resource/pubmed/commentcorrection/11722881-6787018, http://linkedlifedata.com/resource/pubmed/commentcorrection/11722881-7618890, http://linkedlifedata.com/resource/pubmed/commentcorrection/11722881-7747977, http://linkedlifedata.com/resource/pubmed/commentcorrection/11722881-8299952, http://linkedlifedata.com/resource/pubmed/commentcorrection/11722881-8361360, http://linkedlifedata.com/resource/pubmed/commentcorrection/11722881-8366036, http://linkedlifedata.com/resource/pubmed/commentcorrection/11722881-8635755, http://linkedlifedata.com/resource/pubmed/commentcorrection/11722881-8809553, http://linkedlifedata.com/resource/pubmed/commentcorrection/11722881-8879413, http://linkedlifedata.com/resource/pubmed/commentcorrection/11722881-9057296, http://linkedlifedata.com/resource/pubmed/commentcorrection/11722881-9139932, http://linkedlifedata.com/resource/pubmed/commentcorrection/11722881-9178460, http://linkedlifedata.com/resource/pubmed/commentcorrection/11722881-9219268, http://linkedlifedata.com/resource/pubmed/commentcorrection/11722881-9435087, http://linkedlifedata.com/resource/pubmed/commentcorrection/11722881-9488384, http://linkedlifedata.com/resource/pubmed/commentcorrection/11722881-9537390
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
67
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5370-6
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Surface expression of the conserved C repeat region of streptococcal M6 protein within the Pip bacteriophage receptor of Lactococcus lactis.
pubmed:affiliation
Department of Microbiology, Oregon State University, Corvallis, Oregon 97331-3804, USA. gellerb@orst.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't