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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5547
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pubmed:dateCreated |
2001-11-26
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pubmed:databankReference |
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pubmed:abstractText |
We determined a crystal structure of bovine Arp2/3 complex, an assembly of seven proteins that initiates actin polymerization in eukaryotic cells, at 2.0 angstrom resolution. Actin-related protein 2 (Arp2) and Arp3 are folded like actin, with distinctive surface features. Subunits ARPC2 p34 and ARPC4 p20 in the core of the complex associate through long carboxyl-terminal alpha helices and have similarly folded amino-terminal alpha/beta domains. ARPC1 p40 is a seven-blade beta propeller with an insertion that may associate with the side of an actin filament. ARPC3 p21 and ARPC5 p16 are globular alpha-helical subunits. We predict that WASp/Scar proteins activate Arp2/3 complex by bringing Arp2 into proximity with Arp3 for nucleation of a branch on the side of a preexisting actin filament.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0036-8075
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
23
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pubmed:volume |
294
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1679-84
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:11721045-Actin Cytoskeleton,
pubmed-meshheading:11721045-Actin-Related Protein 2,
pubmed-meshheading:11721045-Actin-Related Protein 3,
pubmed-meshheading:11721045-Actins,
pubmed-meshheading:11721045-Adenosine Triphosphate,
pubmed-meshheading:11721045-Animals,
pubmed-meshheading:11721045-Cattle,
pubmed-meshheading:11721045-Crystallography, X-Ray,
pubmed-meshheading:11721045-Cytoskeletal Proteins,
pubmed-meshheading:11721045-Macromolecular Substances,
pubmed-meshheading:11721045-Models, Biological,
pubmed-meshheading:11721045-Models, Molecular,
pubmed-meshheading:11721045-Muscle, Skeletal,
pubmed-meshheading:11721045-Protein Structure, Quaternary,
pubmed-meshheading:11721045-Protein Structure, Secondary,
pubmed-meshheading:11721045-Protein Structure, Tertiary,
pubmed-meshheading:11721045-Protein Subunits,
pubmed-meshheading:11721045-Static Electricity,
pubmed-meshheading:11721045-Thymus Gland
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pubmed:year |
2001
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pubmed:articleTitle |
Crystal structure of Arp2/3 complex.
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pubmed:affiliation |
Structural Biology Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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