Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2001-11-26
pubmed:abstractText
There are 10 known putative pseudouridine synthase genes in Escherichia coli. The products of six have been previously assigned, one to formation of the single pseudouridine in 16S RNA, three to the formation of seven pseudouridines in 23S RNA, and three to the formation of three pseudouridines in tRNA (one synthase makes pseudouridine in 23S RNA and tRNA). Here we show that the remaining four putative synthase genes make bona fide pseudouridine synthases and identify which pseudouridines they make. RluB (formerly YciL) and RluE (formerly YmfC) make pseudouridine2605 and pseudouridine2457, respectively, in 23S RNA. RluF (formerly YjbC) makes the newly discovered pseudouridine2604 in 23S RNA, and TruC (formerly YqcB) makes pseudouridine65 in tRNA(Ile1) and tRNA(Asp). Deletion of each of these synthase genes individually had no effect on exponential growth in rich media at 25 degrees C, 37 degrees C, or 42 degrees C. A strain lacking RluB and RluF also showed no growth defect under these conditions. Mutation of a conserved aspartate in a common sequence motif, previously shown to be essential for the other six E. coli pseudouridine synthases and several yeast pseudouridine synthases, also caused a loss of in vivo activity in all four of the synthases studied in this work.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11720289-10337479, http://linkedlifedata.com/resource/pubmed/commentcorrection/11720289-10339815, http://linkedlifedata.com/resource/pubmed/commentcorrection/11720289-10376875, http://linkedlifedata.com/resource/pubmed/commentcorrection/11720289-10383384, http://linkedlifedata.com/resource/pubmed/commentcorrection/11720289-10428788, http://linkedlifedata.com/resource/pubmed/commentcorrection/11720289-10523634, http://linkedlifedata.com/resource/pubmed/commentcorrection/11720289-10580099, http://linkedlifedata.com/resource/pubmed/commentcorrection/11720289-10756195, http://linkedlifedata.com/resource/pubmed/commentcorrection/11720289-10937990, http://linkedlifedata.com/resource/pubmed/commentcorrection/11720289-10937997, http://linkedlifedata.com/resource/pubmed/commentcorrection/11720289-11142385, http://linkedlifedata.com/resource/pubmed/commentcorrection/11720289-11406626, http://linkedlifedata.com/resource/pubmed/commentcorrection/11720289-11453071, http://linkedlifedata.com/resource/pubmed/commentcorrection/11720289-2187870, http://linkedlifedata.com/resource/pubmed/commentcorrection/11720289-2247610, http://linkedlifedata.com/resource/pubmed/commentcorrection/11720289-2981810, http://linkedlifedata.com/resource/pubmed/commentcorrection/11720289-2991861, http://linkedlifedata.com/resource/pubmed/commentcorrection/11720289-323061, http://linkedlifedata.com/resource/pubmed/commentcorrection/11720289-3276686, http://linkedlifedata.com/resource/pubmed/commentcorrection/11720289-4597452, http://linkedlifedata.com/resource/pubmed/commentcorrection/11720289-4943184, http://linkedlifedata.com/resource/pubmed/commentcorrection/11720289-7489483, http://linkedlifedata.com/resource/pubmed/commentcorrection/11720289-7493321, http://linkedlifedata.com/resource/pubmed/commentcorrection/11720289-7524026, http://linkedlifedata.com/resource/pubmed/commentcorrection/11720289-7612632, http://linkedlifedata.com/resource/pubmed/commentcorrection/11720289-7947756, http://linkedlifedata.com/resource/pubmed/commentcorrection/11720289-8332527, http://linkedlifedata.com/resource/pubmed/commentcorrection/11720289-8373778, http://linkedlifedata.com/resource/pubmed/commentcorrection/11720289-8710514, http://linkedlifedata.com/resource/pubmed/commentcorrection/11720289-8871555, http://linkedlifedata.com/resource/pubmed/commentcorrection/11720289-9047361, http://linkedlifedata.com/resource/pubmed/commentcorrection/11720289-9335267, http://linkedlifedata.com/resource/pubmed/commentcorrection/11720289-9425056, http://linkedlifedata.com/resource/pubmed/commentcorrection/11720289-9660827, http://linkedlifedata.com/resource/pubmed/commentcorrection/11720289-9770678, http://linkedlifedata.com/resource/pubmed/commentcorrection/11720289-9814761, http://linkedlifedata.com/resource/pubmed/commentcorrection/11720289-9843401, http://linkedlifedata.com/resource/pubmed/commentcorrection/11720289-9888802
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1355-8382
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1603-15
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Identification and site of action of the remaining four putative pseudouridine synthases in Escherichia coli.
pubmed:affiliation
Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Florida 33101, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.