Source:http://linkedlifedata.com/resource/pubmed/id/11719848
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2001-11-23
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pubmed:abstractText |
The human epithelial channels, CaT1 and CaT2, were expressed in oocytes, and their single-channel characteristics were compared. In the presence of Na+ and K+ as charge carriers in the pipette solutions, channel activities were observed only when the the extracellular sides of the patches were exposed to nominally Ca2+- and Mg2+-free solutions. In patches of both CaT1- and CaT2-expressing oocytes, multiple channel openings were observed, but the current levels were higher in CaT2-expressing oocytes, particularly at more negative voltages. With K+ as a charge carrier in patches of CaT1-expressing oocytes, the channel activity was low at -10 to -60 mV, but increased dramatically at more negative potentials. This voltage dependence was observed in the presence of both Na+ and K+. The channel activity with Na+, however, was higher at all potentials. Differences between the voltage dependencies for the two cations were also observed in CaT2-expressing oocytes, but the channel activities were higher than those in CaT1-expressing oocytes, particularly in the presence of Na+. We also found that low concentrations of extracellular Mg2+ (5-50 microm) elicited a strong inhibitory action on the CaT channels. Activation of the CaT1 and CaT2 channels by hyperpolarization and other factors may promote increased Ca2+ entry that participates in stimulation of intestinal absorption and renal reabsorption and/or other Ca2+ transport mechanisms in epithelial cells.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acid Transport Systems, Basic,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Magnesium,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/SLC7A2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium,
http://linkedlifedata.com/resource/pubmed/chemical/TRPV Cation Channels,
http://linkedlifedata.com/resource/pubmed/chemical/TRPV6 channel
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0022-2631
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
184
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
113-20
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:11719848-Amino Acid Transport Systems, Basic,
pubmed-meshheading:11719848-Animals,
pubmed-meshheading:11719848-Calcium,
pubmed-meshheading:11719848-Calcium Channels,
pubmed-meshheading:11719848-Epithelial Cells,
pubmed-meshheading:11719848-Humans,
pubmed-meshheading:11719848-Magnesium,
pubmed-meshheading:11719848-Membrane Potentials,
pubmed-meshheading:11719848-Oocytes,
pubmed-meshheading:11719848-Potassium,
pubmed-meshheading:11719848-RNA, Complementary,
pubmed-meshheading:11719848-Rats,
pubmed-meshheading:11719848-Sodium,
pubmed-meshheading:11719848-TRPV Cation Channels,
pubmed-meshheading:11719848-Transfection,
pubmed-meshheading:11719848-Xenopus laevis
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pubmed:year |
2001
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pubmed:articleTitle |
Single-channel activities of the human epithelial Ca2+ transport proteins CaT1 and CaT2.
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pubmed:affiliation |
Endocrine-Hypertension, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, 221 Longwood Ave., Boston, MA 02115, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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