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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2001-11-23
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pubmed:abstractText |
The functional consequences of glycan structural changes associated with cellular differentiation are ill defined. Herein, we investigate the role of glycan adducts to the O-glycosylated polypeptide stalk tethering the CD8alphabeta coreceptor to the thymocyte surface. We show that immature CD4(+)CD8(+) double-positive thymocytes bind MHCI tetramers more avidly than mature CD8 single-positive thymocytes, and that this differential binding is governed by developmentally programmed O-glycan modification controlled by the ST3Gal-I sialyltransferase. ST3Gal-I induction and attendant core 1 sialic acid addition to CD8beta on mature thymocytes decreases CD8alphabeta-MHCI avidity by altering CD8alphabeta domain-domain association and/or orientation. Hence, glycans on the CD8beta stalk appear to modulate the ability of the distal binding surface of the dimeric CD8 globular head domains to clamp MHCI.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD8,
http://linkedlifedata.com/resource/pubmed/chemical/CD8alphabeta antigen,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/H-2 Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/N-Acetylneuraminic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Polysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Rag2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Sialyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/V(D)J recombination activating...,
http://linkedlifedata.com/resource/pubmed/chemical/beta-galactoside...
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
16
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pubmed:volume |
107
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
501-12
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:11719190-Alternative Splicing,
pubmed-meshheading:11719190-Amino Acid Sequence,
pubmed-meshheading:11719190-Animals,
pubmed-meshheading:11719190-Antigens, CD8,
pubmed-meshheading:11719190-CD4-Positive T-Lymphocytes,
pubmed-meshheading:11719190-CD8-Positive T-Lymphocytes,
pubmed-meshheading:11719190-Cell Differentiation,
pubmed-meshheading:11719190-Clonal Deletion,
pubmed-meshheading:11719190-DNA-Binding Proteins,
pubmed-meshheading:11719190-Dimerization,
pubmed-meshheading:11719190-Gene Rearrangement, T-Lymphocyte,
pubmed-meshheading:11719190-Glycosylation,
pubmed-meshheading:11719190-H-2 Antigens,
pubmed-meshheading:11719190-Ligands,
pubmed-meshheading:11719190-Mice,
pubmed-meshheading:11719190-Mice, Inbred C57BL,
pubmed-meshheading:11719190-Mice, Knockout,
pubmed-meshheading:11719190-Models, Molecular,
pubmed-meshheading:11719190-Molecular Sequence Data,
pubmed-meshheading:11719190-N-Acetylneuraminic Acid,
pubmed-meshheading:11719190-Polysaccharides,
pubmed-meshheading:11719190-Protein Binding,
pubmed-meshheading:11719190-Protein Conformation,
pubmed-meshheading:11719190-Protein Processing, Post-Translational,
pubmed-meshheading:11719190-Protein Structure, Tertiary,
pubmed-meshheading:11719190-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:11719190-Recombinant Fusion Proteins,
pubmed-meshheading:11719190-Sequence Alignment,
pubmed-meshheading:11719190-Sequence Homology, Amino Acid,
pubmed-meshheading:11719190-Sialyltransferases,
pubmed-meshheading:11719190-Structure-Activity Relationship,
pubmed-meshheading:11719190-Thymus Gland,
pubmed-meshheading:11719190-Transgenes
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pubmed:year |
2001
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pubmed:articleTitle |
Developmentally regulated glycosylation of the CD8alphabeta coreceptor stalk modulates ligand binding.
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pubmed:affiliation |
Laboratory of Immunobiology, Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Department of Medicine, Harvard Medical School, Boston, MA 02115, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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