Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2001-11-23
pubmed:abstractText
We report here oxidation of propylbenzene and 3-chlorostyrene by wild-type cytochrome P450 BM-3 with high turnover (479 nmol 1-phenyl-1-propanol/min/nmol P450 and 300 nmol 3-chlorostyrene oxide/min/nmol P450). Furthermore, the residue size at position 87 of P450 BM-3 was found to play critical roles in determining stereoselectivity in oxidation of propylbenzene and 3-chlorostyrene. Replacement of Phe87 with Val, Ala and Gly resulted in decreases in optical purity of produced (R)-(+)-1-phenyl-1-propanol from 90.0 to 37.4, 26.0 and -15.6% e.e., respectively, and in increases in those of produced (R)-(+)-3-chlorostyrene oxide from -61.0 to -38.0, 67.0 and 94.6% e.e., respectively.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0014-5793
pubmed:author
pubmed:issnType
Print
pubmed:day
16
pubmed:volume
508
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
249-52
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11718725-Amino Acid Sequence, pubmed-meshheading:11718725-Amino Acid Substitution, pubmed-meshheading:11718725-Bacillus megaterium, pubmed-meshheading:11718725-Bacterial Proteins, pubmed-meshheading:11718725-Benzene Derivatives, pubmed-meshheading:11718725-Cytochrome P-450 Enzyme System, pubmed-meshheading:11718725-Gas Chromatography-Mass Spectrometry, pubmed-meshheading:11718725-Hydroxylation, pubmed-meshheading:11718725-Mixed Function Oxygenases, pubmed-meshheading:11718725-Mutation, pubmed-meshheading:11718725-NADPH-Ferrihemoprotein Reductase, pubmed-meshheading:11718725-Oxidation-Reduction, pubmed-meshheading:11718725-Stereoisomerism, pubmed-meshheading:11718725-Structure-Activity Relationship, pubmed-meshheading:11718725-Styrene, pubmed-meshheading:11718725-Substrate Specificity
pubmed:year
2001
pubmed:articleTitle
Residue size at position 87 of cytochrome P450 BM-3 determines its stereoselectivity in propylbenzene and 3-chlorostyrene oxidation.
pubmed:affiliation
Division of Applied Life Science, Graduate School of Agriculture, Kyoto University, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't