Linked Life Data

1171825

Source:http://linkedlifedata.com/resource/pubmed/id/1171825

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1975-11-26
pubmed:abstractText
Crossed immunoelectrophoresis (CIE) with intermediate gel was applied to the serological analysis of Actinomyces israelii to develop a test with high efficiency in the laboratory diagnosis of human actinomycosis and classification of A. israelii. Recently developed standard antigen-antibody systems for A. israelii by CIE were used as reference. The reference systems were based on standard preparations of cytoplasmic and whole cell-associated antigens of A. israelii and a standard immunoglobulin G pool purified from rabbit antisera to formalin-treated whole cells and cell lysates of A. israelii. The specificity of the standard antigens for A. israelii was evaluated in CIE studies by screening for antibodies to components of the antigens in rabbit antisera raised against related bacteria. The standard system for A. israelii based on cytoplasmic antigens formed species-specific precipitins whereas antisera raised against A. naeslundii and/or Propionibacterium acnes precipitated components of the other standard antigens. As a result of these analyses, the standard system for A. israelii based on 10 cytoplasmic antigens was used as reference for CIE studies to detect humoral antibodies to A. israelii in sera from nine patients with actinomycosis. All the sera from the patients formed at the time of diagnosis one or more precipitins in terms of the 10 reference precipitins. Up to five precipitins were found in single sera. Follow-up studies covering a period of one-half year after treatment showed a gradually decreased precipitin response in the course of time. In control sera from patients with newly diagnosed tuberculosis, nocardiosis, deep Candida infection, and aspergillosis, and in sera from healthy blood donors, no antibodies were detected with specificity for the reference antigens.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1171825-14032869, http://linkedlifedata.com/resource/pubmed/commentcorrection/1171825-14203367, http://linkedlifedata.com/resource/pubmed/commentcorrection/1171825-16560493, http://linkedlifedata.com/resource/pubmed/commentcorrection/1171825-4120179, http://linkedlifedata.com/resource/pubmed/commentcorrection/1171825-4123707, http://linkedlifedata.com/resource/pubmed/commentcorrection/1171825-4151213, http://linkedlifedata.com/resource/pubmed/commentcorrection/1171825-4190518, http://linkedlifedata.com/resource/pubmed/commentcorrection/1171825-4204843, http://linkedlifedata.com/resource/pubmed/commentcorrection/1171825-4207518, http://linkedlifedata.com/resource/pubmed/commentcorrection/1171825-4619788, http://linkedlifedata.com/resource/pubmed/commentcorrection/1171825-4797923, http://linkedlifedata.com/resource/pubmed/commentcorrection/1171825-4886286, http://linkedlifedata.com/resource/pubmed/commentcorrection/1171825-4886287, http://linkedlifedata.com/resource/pubmed/commentcorrection/1171825-4912526, http://linkedlifedata.com/resource/pubmed/commentcorrection/1171825-4964473, http://linkedlifedata.com/resource/pubmed/commentcorrection/1171825-4975155, http://linkedlifedata.com/resource/pubmed/commentcorrection/1171825-5696287, http://linkedlifedata.com/resource/pubmed/commentcorrection/1171825-5937255
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
398-403
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1975
pubmed:articleTitle
Serological studies of Actinomyces israelii by crossed immunoelectrophoresis: taxonomic and diagnostic applications.
pubmed:publicationType
Journal Article