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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
2001-11-21
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pubmed:abstractText |
Toll-like receptor 4 (TLR4) recognizes lipopolysaccharide (LPS). MD-2 is associated with TLR4 and imparts LPS responsiveness to it. Little is known, however, as to whether MD-2 directly regulates LPS recognition by TLR4. To address the issue, we took advantage of a species-specific pharmacology of lipid IVa, an analogue of lipid A. Lipid IVa acted agonistically on mouse (m) TLR4/MD-2 but not on human (h) TLR4/MD-2. Lipid IVa antagonized the agonistic effect of lipid A on hTLR4/MD-2. We examined the chimeric complex consisting of mTLR4 and hMD-2 to ask whether species specificity is conferred by TLR4 or MD-2. hMD-2 was clearly distinct from mMD-2 in the way of influencing LPS recognition by mTLR4. hMD-2 conferred on mTLR4 responsiveness to lipid A but not to lipid IVa. Moreover, lipid IVa acted as a lipid A antagonist on mTLR4 that is associated with hMD-2. Collectively, MD-2 directly influences the fine specificity of TLR4.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adjuvants, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Drosophila Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Glycolipids,
http://linkedlifedata.com/resource/pubmed/chemical/LY96 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Lipid A,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphocyte Antigen 96,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/TLR4 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Toll-Like Receptor 4,
http://linkedlifedata.com/resource/pubmed/chemical/Toll-Like Receptors,
http://linkedlifedata.com/resource/pubmed/chemical/lipid A precursors, bacterial
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0953-8178
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pubmed:author |
pubmed-author:AkashiSS,
pubmed-author:FukaseKK,
pubmed-author:HayashiSS,
pubmed-author:KawasakiKK,
pubmed-author:KimotoMM,
pubmed-author:KusumotoSS,
pubmed-author:MiyakeKK,
pubmed-author:NagaiYY,
pubmed-author:NishijimaMM,
pubmed-author:OgataHH,
pubmed-author:OikawaMM
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pubmed:issnType |
Print
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pubmed:volume |
13
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1595-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11717200-Adjuvants, Immunologic,
pubmed-meshheading:11717200-Animals,
pubmed-meshheading:11717200-Antigens, Surface,
pubmed-meshheading:11717200-Cell Line,
pubmed-meshheading:11717200-Drosophila Proteins,
pubmed-meshheading:11717200-Glycolipids,
pubmed-meshheading:11717200-Humans,
pubmed-meshheading:11717200-Lipid A,
pubmed-meshheading:11717200-Lipopolysaccharides,
pubmed-meshheading:11717200-Lymphocyte Antigen 96,
pubmed-meshheading:11717200-Membrane Glycoproteins,
pubmed-meshheading:11717200-Mice,
pubmed-meshheading:11717200-Receptors, Cell Surface,
pubmed-meshheading:11717200-Species Specificity,
pubmed-meshheading:11717200-Toll-Like Receptor 4,
pubmed-meshheading:11717200-Toll-Like Receptors,
pubmed-meshheading:11717200-Transfection
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pubmed:year |
2001
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pubmed:articleTitle |
Human MD-2 confers on mouse Toll-like receptor 4 species-specific lipopolysaccharide recognition.
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pubmed:affiliation |
Department of Immunology, Saga Medical School, 5-1-1 Nabeshima, Saga 849-8501, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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