Linked Life Data

11714817

Source:http://linkedlifedata.com/resource/pubmed/id/11714817

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2001-11-20
pubmed:abstractText
We investigated whether secretion of multiple cytokines by CD8+ T cells is associated with improved protection against tumor challenge. We show that antitumor immunity induced by immunization with dendritic cells and a MHC class I-binding tumor peptide are dependent on secretion of IFN-gamma but not IL-4 or IL-5 by host cells. To further address the role of IL-4 and IL-5 in antitumor immunity, tumor-specific TCR-transgenic CD8+ T cells were activated in vitro to generate cytotoxic T (Tc) 1 cells that secrete high IFN-gamma and no IL-4 or IL-5 or Tc2 cells that secrete IL-4, IL-5, and some IFN-gamma. Both cell types killed target cells in vitro. Tc1 and Tc2 cells were adoptively transferred into syngeneic hosts, and their ability to protect against tumor challenge was compared. Tc1 cells were able to significantly delay tumor growth, whereas Tc2 cells or Tc2 cells from IFN-gamma(-/-) donors had no effect. This was due to neither the inability of Tc2 cells to survive in vivo or to migrate to the tumor site nor their inability to secrete IL-4 and/or IL-5 in the presence of limiting amounts of anti-CD3. However, IFN-gamma secretion by Tc2 cells was triggered inefficiently by restimulation with Ag compared with anti-CD3. We conclude that the ability to secrete "type 2" cytokines, and cytotoxic ability, have a limited role in antitumor immune responses mediated by CD8+ T cells, whereas the capacity to secrete high amounts of IFN-gamma remains the most critical antitumor effector mechanism in vivo.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
167
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6497-502
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11714817-Animals, pubmed-meshheading:11714817-Antigens, Neoplasm, pubmed-meshheading:11714817-CD8-Positive T-Lymphocytes, pubmed-meshheading:11714817-Carcinoma, Lewis Lung, pubmed-meshheading:11714817-Cell Movement, pubmed-meshheading:11714817-Cell Survival, pubmed-meshheading:11714817-Cells, Cultured, pubmed-meshheading:11714817-Cytokines, pubmed-meshheading:11714817-Dendritic Cells, pubmed-meshheading:11714817-Epitopes, T-Lymphocyte, pubmed-meshheading:11714817-Female, pubmed-meshheading:11714817-Graft Rejection, pubmed-meshheading:11714817-Histocompatibility Antigens Class I, pubmed-meshheading:11714817-Immunotherapy, Adoptive, pubmed-meshheading:11714817-Injections, Subcutaneous, pubmed-meshheading:11714817-Interferon-gamma, pubmed-meshheading:11714817-Interleukin-4, pubmed-meshheading:11714817-Interleukin-5, pubmed-meshheading:11714817-Ligands, pubmed-meshheading:11714817-Lymphocyte Activation, pubmed-meshheading:11714817-Male, pubmed-meshheading:11714817-Mice, pubmed-meshheading:11714817-Mice, Inbred C57BL, pubmed-meshheading:11714817-Mice, Knockout, pubmed-meshheading:11714817-Mice, Transgenic, pubmed-meshheading:11714817-Peptide Fragments, pubmed-meshheading:11714817-Protein Binding, pubmed-meshheading:11714817-Receptors, Antigen, T-Cell, pubmed-meshheading:11714817-T-Lymphocyte Subsets, pubmed-meshheading:11714817-T-Lymphocytes, Cytotoxic, pubmed-meshheading:11714817-Tumor Cells, Cultured
pubmed:year
2001
pubmed:articleTitle
Tumor-specific Tc1, but not Tc2, cells deliver protective antitumor immunity.
pubmed:affiliation
Malaghan Institute of Medical Research, Wellington School of Medicine, Wellington, New Zealand.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't