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rdf:type |
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lifeskim:mentions |
umls-concept:C0004587,
umls-concept:C0024518,
umls-concept:C0031310,
umls-concept:C0291677,
umls-concept:C0547047,
umls-concept:C1254042,
umls-concept:C1314939,
umls-concept:C1522492,
umls-concept:C1548795,
umls-concept:C1704241,
umls-concept:C1709694,
umls-concept:C2263441
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pubmed:issue |
10
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pubmed:dateCreated |
2001-11-20
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pubmed:abstractText |
Mycobacterium tuberculosis (MTB) inhibits phagosomal maturation to promote its survival inside macrophages. Control of MTB infection requires CD4 T cell responses and major histocompatibility complex (MHC) class II (MHC-II) processing of MTB antigens (Ags). To investigate phagosomal processing of MTB Ags, phagosomes containing heat-killed (HK) or live MTB were purified from interferon-gamma (IFN-gamma)-activated macrophages by differential centrifugation and Percoll density gradient subcellular fractionation. Flow organellometry and Western blot analysis showed that MTB phagosomes acquired lysosome-associated membrane protein-1 (LAMP-1), MHC-II, and H2-DM. T hybridoma cells were used to detect MTB Ag 85B(241-256)-I-A(b) complexes in isolated phagosomes and other subcellular fractions. These complexes appeared initially (within 20 min) in phagosomes and subsequently (>20 min) on the plasma membrane, but never within late endocytic compartments. Macrophages processed HK MTB more rapidly and efficiently than live MTB; phagosomes containing live MTB expressed fewer Ag 85B(241-256)-I-A(b) complexes than phagosomes containing HK MTB. This is the first study of bacterial Ag processing to directly show that peptide-MHC-II complexes are formed within phagosomes and not after export of bacterial Ags from phagosomes to endocytic Ag processing compartments. Live MTB can alter phagosome maturation and decrease MHC-II Ag processing, providing a mechanism for MTB to evade immune surveillance and enhance its survival within the host.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-10092778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-10338208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-10399077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-10438942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-10453010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-10569246,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-10768959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-10799867,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-10805975,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-11086039,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-11441098,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-1480113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-15776571,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-1623927,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-1847504,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-1898911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-1904624,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-2030721,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-2464638,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-2909613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-3877730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-7600287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-7807006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-7836763,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-7909813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-8039918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-8077667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-8098550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-8177316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-8177317,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-8177319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-8288311,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-8303277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-8409381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-8557358,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-8575463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-8598037,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-8876215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-8879207,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-9029086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-9119492,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-9162010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-9348281,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-9490634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-9570546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-9671125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11714749-9794422
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/H2-M antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Histocompatibility Antigens Class II,
http://linkedlifedata.com/resource/pubmed/chemical/Lysosome-Associated Membrane...,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/antigen 85B, Mycobacterium...,
http://linkedlifedata.com/resource/pubmed/chemical/invariant chain
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
19
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pubmed:volume |
194
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1421-32
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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