Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 1
pubmed:dateCreated
2001-11-16
pubmed:abstractText
1. Large-conductance Ca(2+)- and voltage-activated potassium (BK) channels are important regulators of cellular excitability. Here, we present a patch-clamp electrophysiological analysis of splice-variant-specific regulation by the synthetic glucocorticoid dexamethasone (DEX) of BK channels consisting of cloned STREX or ZERO alpha-subunit variants expressed in human embryonic kidney (HEK 293) cells. 2. STREX channels in isolated membrane patches were inhibited by protein kinase A (PKA) and this was blocked on pre-treatment of intact cells with DEX (100 nM) for 2 h. 3. The effect of DEX required the synthesis of new mRNA and protein. Furthermore, it required protein phosphatase 2A (PP2A)-like activity intimately associated with the channels, as it was blocked by 10 nM okadaic acid but not by the specific protein phosphatase-1 inhibitor peptide PPI-2. 4. ZERO variant channels that lack the STREX insert were activated by PKA but were not influenced by DEX. ZERO channels containing a mutant STREX domain (S4(STREX)A) were also activated by PKA. Importantly, DEX blocked PKA activation of S4(STREX)A channels in a PP2A-dependent manner. 5. Taken together, the STREX domain is crucial for glucocorticoid regulation of BK channels through a PP2A-type enzyme. Moreover, glucocorticoids appear to induce a generic set of proteins in different types of cells, the actions of which depend on the expression of cell-specific targets.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11711561-10066921, http://linkedlifedata.com/resource/pubmed/commentcorrection/11711561-10200423, http://linkedlifedata.com/resource/pubmed/commentcorrection/11711561-10517674, http://linkedlifedata.com/resource/pubmed/commentcorrection/11711561-10696570, http://linkedlifedata.com/resource/pubmed/commentcorrection/11711561-10766916, http://linkedlifedata.com/resource/pubmed/commentcorrection/11711561-10871603, http://linkedlifedata.com/resource/pubmed/commentcorrection/11711561-11309619, http://linkedlifedata.com/resource/pubmed/commentcorrection/11711561-11331373, http://linkedlifedata.com/resource/pubmed/commentcorrection/11711561-1332850, http://linkedlifedata.com/resource/pubmed/commentcorrection/11711561-1497890, http://linkedlifedata.com/resource/pubmed/commentcorrection/11711561-1646298, http://linkedlifedata.com/resource/pubmed/commentcorrection/11711561-1710783, http://linkedlifedata.com/resource/pubmed/commentcorrection/11711561-1791837, http://linkedlifedata.com/resource/pubmed/commentcorrection/11711561-2781292, http://linkedlifedata.com/resource/pubmed/commentcorrection/11711561-2867473, http://linkedlifedata.com/resource/pubmed/commentcorrection/11711561-7515569, http://linkedlifedata.com/resource/pubmed/commentcorrection/11711561-7588309, http://linkedlifedata.com/resource/pubmed/commentcorrection/11711561-7597086, http://linkedlifedata.com/resource/pubmed/commentcorrection/11711561-7687074, http://linkedlifedata.com/resource/pubmed/commentcorrection/11711561-7769088, http://linkedlifedata.com/resource/pubmed/commentcorrection/11711561-7790924, http://linkedlifedata.com/resource/pubmed/commentcorrection/11711561-7987297, http://linkedlifedata.com/resource/pubmed/commentcorrection/11711561-7993625, http://linkedlifedata.com/resource/pubmed/commentcorrection/11711561-8621576, http://linkedlifedata.com/resource/pubmed/commentcorrection/11711561-8858210, http://linkedlifedata.com/resource/pubmed/commentcorrection/11711561-9115223, http://linkedlifedata.com/resource/pubmed/commentcorrection/11711561-9545224, http://linkedlifedata.com/resource/pubmed/commentcorrection/11711561-9593688, http://linkedlifedata.com/resource/pubmed/commentcorrection/11711561-9614096
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP-Dependent Protein Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Dexamethasone, http://linkedlifedata.com/resource/pubmed/chemical/Glucocorticoids, http://linkedlifedata.com/resource/pubmed/chemical/Large-Conductance..., http://linkedlifedata.com/resource/pubmed/chemical/Large-Conductance..., http://linkedlifedata.com/resource/pubmed/chemical/Phosphoprotein Phosphatases, http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channel Blockers, http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channels, http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channels..., http://linkedlifedata.com/resource/pubmed/chemical/Protein Isoforms, http://linkedlifedata.com/resource/pubmed/chemical/Protein Phosphatase 1, http://linkedlifedata.com/resource/pubmed/chemical/Protein Phosphatase 2, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Glucocorticoid
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-3751
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
537
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
57-68
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:11711561-Alternative Splicing, pubmed-meshheading:11711561-Animals, pubmed-meshheading:11711561-Cell Line, pubmed-meshheading:11711561-Cyclic AMP-Dependent Protein Kinases, pubmed-meshheading:11711561-Dexamethasone, pubmed-meshheading:11711561-Glucocorticoids, pubmed-meshheading:11711561-Humans, pubmed-meshheading:11711561-Large-Conductance Calcium-Activated Potassium Channel..., pubmed-meshheading:11711561-Large-Conductance Calcium-Activated Potassium Channels, pubmed-meshheading:11711561-Mice, pubmed-meshheading:11711561-Phosphoprotein Phosphatases, pubmed-meshheading:11711561-Potassium Channel Blockers, pubmed-meshheading:11711561-Potassium Channels, pubmed-meshheading:11711561-Potassium Channels, Calcium-Activated, pubmed-meshheading:11711561-Protein Biosynthesis, pubmed-meshheading:11711561-Protein Isoforms, pubmed-meshheading:11711561-Protein Phosphatase 1, pubmed-meshheading:11711561-Protein Phosphatase 2, pubmed-meshheading:11711561-Protein Structure, Tertiary, pubmed-meshheading:11711561-RNA, Messenger, pubmed-meshheading:11711561-Receptors, Glucocorticoid
pubmed:year
2001
pubmed:articleTitle
Alternative splicing determines sensitivity of murine calcium-activated potassium channels to glucocorticoids.
pubmed:affiliation
Membrane Biology Group and MRC "Membrane and Adapter Protein" COOP, Section of Biomedical Sciences, University of Edinburgh Medical School, Hugh Robson Building, Edinburgh EH8 9XD, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't