Linked Life Data

11710042

Source:http://linkedlifedata.com/resource/pubmed/id/11710042

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2001-11-16
pubmed:abstractText
Thermoresponsive hyaluronans (HAs) were prepared by graft polymerization of N-isopropylacrylamide (NIPAM) on HA (number-averaged molecular weight, Ma, ca. 1.5 x 10(5) and 5.0 x 10(5)) using dithiocarbamate which is a kind of iniferter (initiator, transfer agent and terminator). The degree of dithiocarbamylation (DD) as an iniferter ranged from 0.4 to 11.4% per disaccharide unit of HA. The estimated Mn of the grafted polyNIPAM (PNIPAM) ranged from approximately 5.0 x 10(3) to 8.4 x 10(4). The PNIPAM-grafted HAs (PNIPAM-HAs) were water-soluble at room temperature, while they precipitated at temperatures above approximately 34 degrees C in water. The temperature at the onset of precipitation (lower critical solution temperature: LCST) was independent of parameters of molecular architecture such as Mn of HA, degree of grafting of PNIPAM, and Mn of PNIPAM. Equilibrium transmittance of the aqueous solution above LCST decreased with an increase in both degree of grafting and Mn of PNIPAM. At physiological temperature, the PNIPAM-HA film cast from a cold solution was very wettable with water. A markedly reduced adhesion of endothelial cells to the film was observed, indicating that the PNIPAM-HA film may serve as a non-cell-adhesive matrix. Scanning electron microscopic observation appeared to differentiate supramolecular structures between rapidly freeze-dried PNIPAM-HA and nongrafted HA:PNIPAM-HA exhibited a nonuniform fibrous network, whereas the morphology of which is markedly different from that of a nongrafted HA gel exhibited a mixture of sharp needle- and platelike structures.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1525-7797
pubmed:author
pubmed:issnType
Print
pubmed:volume
2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
856-63
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Thermoresponsive artificial extracellular matrix for tissue engineering: hyaluronic acid bioconjugated with poly(N-isopropylacrylamide) grafts.
pubmed:affiliation
Department of Bioengineering, National Cardiovascular Center Research Institute, Suita, Osaka 565-8565, Japan.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't