Linked Life Data

11709068

Source:http://linkedlifedata.com/resource/pubmed/id/11709068

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 6
pubmed:dateCreated
2001-11-15
pubmed:abstractText
The asymmetrical distribution of neurotransmitter transporters on the cell surface of neurons seems to be a generalized feature of these proteins, and is thought to be important for an appropriate removal of neurotransmitters from the extracellular milieu. To study the subcellular distribution of the glycine transporter isoforms (GLYT1a, GLYT1b, GLYT2a and GLYT2b), these proteins were expressed in epithelial cells [Madin-Darby canine kidney (MDCK) cells] and in cultured hippocampal neurons, as models of polarized cells. The localization of the transporters was assessed by immunofluorescence assays. Our results indicated that the subcellular distribution of glycine transporters is dependent on both the protein isoform and the cell type. By using site-directed mutagenesis we have been able to identify signals for basolateral/somatodendritic localization in the alternative amino terminal region of GLYT1 and in two di-leucine motifs that are located in the carboxyl tail of this protein. Moreover, the N-glycosylation sites located in the large extracellular loop of GLYT2 are involved in apical localization of this protein in polarized MDCK cells. These results contribute to define the mechanisms of asymmetrical distribution of transporters on the cell surface of polarized cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0300-5127
pubmed:author
pubmed:issnType
Print
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
746-50
pubmed:dateRevised
2005-11-17
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Molecular determinants involved in the asymmetrical distribution of glycine transporters in polarized cells.
pubmed:affiliation
Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid, 28049 Madrid, Spain. fzafra@cbm.uam.es
pubmed:publicationType
Journal Article, Review