Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2001-11-13
pubmed:abstractText
We investigated the importance of the host complement system in the pathogenesis of disease mediated by the intramacrophage pathogen Mycobacterium avium. Mycobacteria opsonized with complement are efficiently ingested by macrophages through various complement receptors. Furthermore, unlike other bacteria, mycobacteria can activate both the alternative and classical complement pathways in the absence of specific antibodies. Therefore, to examine the role of complement in the mycobacterial infection process in vivo, mice deficient in complement component C3 were infected with M. avium. Surprisingly, C3-deficient mice infected intravenously with M. avium displayed no difference in bacterial burden or granulomatous response compared to wild-type control mice. C3-sufficient mice and C3-deficient mice were equally susceptible to infection by M. avium regardless of the genotype at the bcg locus, a locus known to confer susceptibility to infection with intracellular pathogens. In vitro studies using mouse bone marrow-derived macrophages resulted in significant M. avium invasion of macrophages in the absence of C3; however, the kinetics of infection were delayed compared to complement-mediated invasion. The data indicate that complement does not play an essential role in mediating M. avium infections in the mouse and suggest either that other invasion mechanisms can compensate for the absence of complement-mediated entry or that complement is not a major mycobacterial opsonin in vivo.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11705954-10352292, http://linkedlifedata.com/resource/pubmed/commentcorrection/11705954-10377141, http://linkedlifedata.com/resource/pubmed/commentcorrection/11705954-10456949, http://linkedlifedata.com/resource/pubmed/commentcorrection/11705954-10594973, http://linkedlifedata.com/resource/pubmed/commentcorrection/11705954-10698336, http://linkedlifedata.com/resource/pubmed/commentcorrection/11705954-10946287, http://linkedlifedata.com/resource/pubmed/commentcorrection/11705954-11013008, http://linkedlifedata.com/resource/pubmed/commentcorrection/11705954-11078603, http://linkedlifedata.com/resource/pubmed/commentcorrection/11705954-11244032, http://linkedlifedata.com/resource/pubmed/commentcorrection/11705954-1679838, http://linkedlifedata.com/resource/pubmed/commentcorrection/11705954-2108212, http://linkedlifedata.com/resource/pubmed/commentcorrection/11705954-2138634, http://linkedlifedata.com/resource/pubmed/commentcorrection/11705954-2958962, http://linkedlifedata.com/resource/pubmed/commentcorrection/11705954-8262640, http://linkedlifedata.com/resource/pubmed/commentcorrection/11705954-8881745, http://linkedlifedata.com/resource/pubmed/commentcorrection/11705954-9129080, http://linkedlifedata.com/resource/pubmed/commentcorrection/11705954-9197241, http://linkedlifedata.com/resource/pubmed/commentcorrection/11705954-9262476, http://linkedlifedata.com/resource/pubmed/commentcorrection/11705954-9317035, http://linkedlifedata.com/resource/pubmed/commentcorrection/11705954-9507637, http://linkedlifedata.com/resource/pubmed/commentcorrection/11705954-9529042, http://linkedlifedata.com/resource/pubmed/commentcorrection/11705954-9596746, http://linkedlifedata.com/resource/pubmed/commentcorrection/11705954-9779380, http://linkedlifedata.com/resource/pubmed/commentcorrection/11705954-9843995
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
69
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7729-35
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:11705954-Animals, pubmed-meshheading:11705954-Antibodies, Bacterial, pubmed-meshheading:11705954-Antibody Specificity, pubmed-meshheading:11705954-Bone Marrow Cells, pubmed-meshheading:11705954-Complement C3, pubmed-meshheading:11705954-Complement Pathway, Alternative, pubmed-meshheading:11705954-Complement Pathway, Classical, pubmed-meshheading:11705954-Disease Susceptibility, pubmed-meshheading:11705954-Granuloma, pubmed-meshheading:11705954-Liver, pubmed-meshheading:11705954-Macrophages, pubmed-meshheading:11705954-Mice, pubmed-meshheading:11705954-Mice, Mutant Strains, pubmed-meshheading:11705954-Mycobacterium avium, pubmed-meshheading:11705954-Opsonin Proteins, pubmed-meshheading:11705954-Spleen, pubmed-meshheading:11705954-Tuberculosis, pubmed-meshheading:11705954-Tumor Necrosis Factor-alpha
pubmed:year
2001
pubmed:articleTitle
Role of complement in Mycobacterium avium pathogenesis: in vivo and in vitro analyses of the host response to infection in the absence of complement component C3.
pubmed:affiliation
Department of Biology, University of Notre Dame, Notre Dame, Indiana 46617, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't