11704539

pubmed:Citation

6

Numerous in vitro and in vivo studies have implicated the cytokines interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha) as mediators of airway inflammation and therefore potentially important substances in the pathogenesis of asthma. In this study, we examined the mechanisms by which IL-1 beta and TNF-alpha affect inhibition of cell growth, G protein-coupled receptor (GPCR) desensitization, and the recently reported adenylyl cyclase sensitization in human airway smooth muscle (HASM) cultures. Our findings demonstrate that adenylyl cyclase sensitization is independent of cytokine-mediated cyclooxygenase type 2 (COX-2) and prostaglandin E(2) (PGE(2)) induction, whereas COX-2 induction appears to be required for both growth inhibition and GPCR desensitization. However, GPCR desensitization was highly dependent on the presence of EGF during chronic treatment with cytokines, which could be explained by a synergistic effect of EGF on cytokine-mediated COX-2 and PGE(2) induction. Interestingly, various agents (including inhibitors of p42/p44 and p38 mitogen-activated protein kinase signaling) were significantly more effective in inhibiting cytokine-mediated PGE(2) induction, GPCR desensitization, and cell growth inhibition than in inhibiting COX-2 induction. These data demonstrate disparity in the requirement and sufficiency of COX-2 induction in promoting different functional effects of IL-1 beta and TNF-alpha in HASM.

eng

IM

MEDLINE

1040-0605

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NLM

L1425-35

pubmed-meshheading:11704539-Adenylate Cyclase, pubmed-meshheading:11704539-Butadienes, pubmed-meshheading:11704539-Cell Division, pubmed-meshheading:11704539-Cells, Cultured, pubmed-meshheading:11704539-Cyclooxygenase 2, pubmed-meshheading:11704539-Enzyme Inhibitors, pubmed-meshheading:11704539-Epidermal Growth Factor, pubmed-meshheading:11704539-GTP-Binding Proteins, pubmed-meshheading:11704539-Humans, pubmed-meshheading:11704539-Imidazoles, pubmed-meshheading:11704539-Interleukin-1, pubmed-meshheading:11704539-Isoenzymes, pubmed-meshheading:11704539-JNK Mitogen-Activated Protein Kinases, pubmed-meshheading:11704539-MAP Kinase Kinase 4, pubmed-meshheading:11704539-MAP Kinase Signaling System, pubmed-meshheading:11704539-Membrane Proteins, pubmed-meshheading:11704539-Mitogen-Activated Protein Kinase 1, pubmed-meshheading:11704539-Mitogen-Activated Protein Kinase 3, pubmed-meshheading:11704539-Mitogen-Activated Protein Kinase Kinases, pubmed-meshheading:11704539-Mitogen-Activated Protein Kinases, pubmed-meshheading:11704539-Muscle, Smooth, pubmed-meshheading:11704539-Nitriles, pubmed-meshheading:11704539-Prostaglandin-Endoperoxide Synthases, pubmed-meshheading:11704539-Pyridines, pubmed-meshheading:11704539-Receptors, Cell Surface, pubmed-meshheading:11704539-Trachea, pubmed-meshheading:11704539-Tumor Necrosis Factor-alpha, pubmed-meshheading:11704539-p38 Mitogen-Activated Protein Kinases

Mechanisms of cytokine effects on G protein-coupled receptor-mediated signaling in airway smooth muscle.

Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't