Source:http://linkedlifedata.com/resource/pubmed/id/11703071
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2001-11-12
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| pubmed:abstractText |
Vasoactive intestinal peptide (VIP) is a prolactin (PRL)-releasing factor whose activity in avian species is believed to be mediated by a specific VIP receptor (VIP-R). Circulating PRL levels are closely related to hypothalamic VIP immunoreactivity, hypothalamic VIP mRNA content, and hypophysial-portal blood VIP concentrations in turkeys. In the present study, a turkey VIP-R (tVIP-R) cDNA was cloned and its mRNA abundance was quantified in various tissues during different reproductive stages. The 2347-bp tVIP-R cDNA encoded a 457 amino acid protein, with a predicted Mr of 52 kDa. The full-length cDNA shares approximately 55% similarity with the mammalian VIP receptor-1. Northern blot analysis revealed that a major 2.7-kb transcript was expressed in laying hen pituitaries. Furthermore, two minor tVIP-R transcripts of 3.7 and 3.4 kb were observed. Semiquantitative reverse transcription polymerase chain reaction (RT-PCR) was performed using RNA from various turkey brain and peripheral tissues throughout the reproductive cycle. The steady-state levels of pituitary tVIP-R mRNA changed during the reproductive cycle, whereas mRNA expression in other tissues was not affected. The steady-state levels of tVIP-R mRNA were only affected in the pituitary, whereas mRNA expression in any of the other tissues examined following the immunization of turkeys against VIP were not affected.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Oct
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| pubmed:issn |
0016-6480
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2001 Academic Press.
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| pubmed:issnType |
Print
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| pubmed:volume |
124
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
53-65
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| pubmed:dateRevised |
2003-11-14
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| pubmed:meshHeading |
pubmed-meshheading:11703071-Amino Acid Sequence,
pubmed-meshheading:11703071-Animals,
pubmed-meshheading:11703071-Base Sequence,
pubmed-meshheading:11703071-Blotting, Northern,
pubmed-meshheading:11703071-Cloning, Molecular,
pubmed-meshheading:11703071-DNA, Complementary,
pubmed-meshheading:11703071-Female,
pubmed-meshheading:11703071-Gene Expression Regulation,
pubmed-meshheading:11703071-Gene Library,
pubmed-meshheading:11703071-Hypothalamus,
pubmed-meshheading:11703071-Intestine, Small,
pubmed-meshheading:11703071-Molecular Sequence Data,
pubmed-meshheading:11703071-Pituitary Gland,
pubmed-meshheading:11703071-Prolactin,
pubmed-meshheading:11703071-RNA, Messenger,
pubmed-meshheading:11703071-Receptors, Vasoactive Intestinal Peptide,
pubmed-meshheading:11703071-Reproduction,
pubmed-meshheading:11703071-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11703071-Turkeys
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| pubmed:year |
2001
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| pubmed:articleTitle |
Molecular cloning and expression analysis of the turkey vasoactive intestinal peptide receptor.
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| pubmed:affiliation |
School of Agricultural Biotechnology, Seoul National University, Suwon 441-744, Korea.
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| pubmed:publicationType |
Journal Article
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