Linked Life Data

11702708

Source:http://linkedlifedata.com/resource/pubmed/id/11702708

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2001-11-12
pubmed:abstractText
During synthesis of GSH by the engineered strain E. coli BL21(pTrc-gsh) coupled with Saccharomyces cerevisiae producing ATP from adenosin, the inconsistency of two systems in the concentration of phosphate buffer was solved by decreasing concentration to 250 mmol/L. The conditions under 250 mmol/L phosphate buffer were optimized and the yield of GSH was 1.6 g/L, which was higher than that of summation by two systems under the same conditions respectively. Addition of glycine later after glutamate and cysteine weakened the inhibition of GSH to GSHI. It made the yield of GSH reach to 2.13 g/L which was 30.7% higher than the control.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1000-3061
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
452-5
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
[Studies on synthesis of glutathione by E. coli BL21 (pTrc-gsh) coupled with Saccharomyces cerevisiae].
pubmed:affiliation
State Key Laboratory of Bioreactor Engineering, Institute of Biochemistry, ECUST, Shanghai 200237, China.
pubmed:publicationType
Journal Article, English Abstract