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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
2001-11-9
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pubmed:databankReference |
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pubmed:abstractText |
The potato pathogenesis-related gene PR-10a is transcriptionally activated in response to pathogen infection or elicitor treatment. Characterization of the cis-acting elements of the PR-10a promoter revealed the presence of a silencing element between residues -52 and -27 that contributes to transcriptional regulation. In this study, we have isolated a silencing element binding factor (SEBF) from potato tuber nuclei that binds to the coding strand of the silencing element in a sequence-specific manner. The consensus binding site of SEBF, PyTGTCNC, is present in a number of PR genes and shows striking similarity to the auxin response element. Mutational analysis of the PR-10a promoter revealed an inverse correlation between the in vitro binding of SEBF and the expression of PR-10a. SEBF was purified to homogeneity from potato tubers, and sequencing of the N terminus of the protein led to the isolation of a cDNA clone. Sequence analysis revealed that SEBF is homologous with chloroplast RNA binding proteins that possess consensus sequence-type RNA binding domains characteristic of heterogeneous nuclear ribonucleoproteins (hnRNPs). Overexpression of SEBF in protoplasts repressed the activity of a PR-10a reporter construct in a silencing element-dependent manner, confirming the role of SEBF as a transcriptional repressor.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-10318972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-10556512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-10794726,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-10891285,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-10948264,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-10972977,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-11038367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-11090213,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-11230137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-1341899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-1398104,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-1406585,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-1433497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-1497306,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-16654194,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-16667307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-16668208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-1721701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-2251262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-2314235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-2520162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-2535551,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-2847873,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-3047011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-3061875,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-7580254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-7630716,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-7764876,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-7876087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-7894017,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-8036511,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-8626459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-8649373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-8806406,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-8999813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-9281422,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-9349252,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-9770453,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-9813044,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11701886-9869429
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Single-Stranded,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/PBF-2 protein, Solanum tuberosum,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1040-4651
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
13
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2525-37
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pubmed:dateRevised |
2010-9-14
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pubmed:meshHeading |
pubmed-meshheading:11701886-Amino Acid Sequence,
pubmed-meshheading:11701886-Base Sequence,
pubmed-meshheading:11701886-Cell Nucleus,
pubmed-meshheading:11701886-Chloroplasts,
pubmed-meshheading:11701886-Cloning, Molecular,
pubmed-meshheading:11701886-DNA, Single-Stranded,
pubmed-meshheading:11701886-DNA Primers,
pubmed-meshheading:11701886-DNA-Binding Proteins,
pubmed-meshheading:11701886-Gene Expression Regulation, Plant,
pubmed-meshheading:11701886-Molecular Sequence Data,
pubmed-meshheading:11701886-Nuclear Proteins,
pubmed-meshheading:11701886-Plant Leaves,
pubmed-meshheading:11701886-Plant Proteins,
pubmed-meshheading:11701886-Polymerase Chain Reaction,
pubmed-meshheading:11701886-Promoter Regions, Genetic,
pubmed-meshheading:11701886-RNA-Binding Proteins,
pubmed-meshheading:11701886-Recombinant Proteins,
pubmed-meshheading:11701886-Repressor Proteins,
pubmed-meshheading:11701886-Sequence Alignment,
pubmed-meshheading:11701886-Solanum tuberosum,
pubmed-meshheading:11701886-Transcription, Genetic
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pubmed:year |
2001
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pubmed:articleTitle |
Repression of the defense gene PR-10a by the single-stranded DNA binding protein SEBF.
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pubmed:affiliation |
Department of Biochemistry, Université de Montréal, Montréal, Québec, Canada H3C 3J7.
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