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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6860
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pubmed:dateCreated |
2001-11-8
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pubmed:databankReference |
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pubmed:abstractText |
The tripartite toxin secreted by Bacillus anthracis, the causative agent of anthrax, helps the bacterium evade the immune system and can kill the host during a systemic infection. Two components of the toxin enzymatically modify substrates within the cytosol of mammalian cells: oedema factor (OF) is an adenylate cyclase that impairs host defences through a variety of mechanisms including inhibiting phagocytosis; lethal factor (LF) is a zinc-dependent protease that cleaves mitogen-activated protein kinase kinase and causes lysis of macrophages. Protective antigen (PA), the third component, binds to a cellular receptor and mediates delivery of the enzymatic components to the cytosol. Here we describe the cloning of the human PA receptor using a genetic complementation approach. The receptor, termed ATR (anthrax toxin receptor), is a type I membrane protein with an extracellular von Willebrand factor A domain that binds directly to PA. In addition, a soluble version of this domain can protect cells from the action of the toxin.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0028-0836
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
8
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pubmed:volume |
414
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
225-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11700562-Amino Acid Sequence,
pubmed-meshheading:11700562-Animals,
pubmed-meshheading:11700562-Antigens, Bacterial,
pubmed-meshheading:11700562-Bacillus anthracis,
pubmed-meshheading:11700562-Bacterial Toxins,
pubmed-meshheading:11700562-CHO Cells,
pubmed-meshheading:11700562-Cloning, Molecular,
pubmed-meshheading:11700562-Cricetinae,
pubmed-meshheading:11700562-Genetic Complementation Test,
pubmed-meshheading:11700562-HeLa Cells,
pubmed-meshheading:11700562-Humans,
pubmed-meshheading:11700562-Molecular Sequence Data,
pubmed-meshheading:11700562-Protein Binding,
pubmed-meshheading:11700562-Protein Structure, Tertiary,
pubmed-meshheading:11700562-Receptors, Peptide,
pubmed-meshheading:11700562-Sequence Alignment,
pubmed-meshheading:11700562-von Willebrand Factor
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pubmed:year |
2001
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pubmed:articleTitle |
Identification of the cellular receptor for anthrax toxin.
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pubmed:affiliation |
McArdle Laboratory for Cancer Research, University of Wisconsin-Madison, 1400 University Avenue, Madison, Wisconsin 53706, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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