Source:http://linkedlifedata.com/resource/pubmed/id/11697505
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2001-11-7
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| pubmed:abstractText |
The persistence of the AML1-ETO rearrangement performed by reverse transcription polymerase chain reaction (RT-PCR) has been reported in acute myeloid leukemia (AML) patients in long-term complete remission (CR). This persistence, which is not associated with hematological relapse, limits the clinical use of qualitative RT-PCR. Here, we present a new quantitative real-time PCR method to detect AML1-ETO rearrangement using fluorescently labeled probes. Quantitative detection of AML1-ETO was performed in capillary tubes using two fluorescently labeled probes in the LightCycler equipment. The reliability of the method was checked in twenty-two bone marrow samples and one apheresis sample from eight patients with t(8;21) collected at diagnosis and during follow-up assessment. The regression coefficients obtained for standard curves of AML1-ETO and AML were all greater than 0.98. The sensitivity attained allowed the detection of rearrangements at a dilution of 10(-5) Kasumi-1 cDNA. The intra-assay coefficient of variation was 4% for AML1-ETO, and 7% for AML. The inter-assay coefficient of variation was 19% for AML1-ETO and 12% for AML. A log reduction from two to four in the AML1-ETO/AML ratio was evident after CR. The study of the method and first results obtained in patient samples support that quantitative real-time PCR with hybridization probes is a new reliable and sensitive method to monitor minimal residual disease in AML patients. Moreover, the fluorescent probes with the Light-Cycler technology offer the advantage of a rapid detection.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/AML1-ETO fusion protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Core Binding Factor Alpha 2 Subunit,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Probes,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Oncogene Proteins, Fusion,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
1042-8194
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
42
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
747-56
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:11697505-Acute Disease,
pubmed-meshheading:11697505-Adolescent,
pubmed-meshheading:11697505-Adult,
pubmed-meshheading:11697505-Child,
pubmed-meshheading:11697505-Child, Preschool,
pubmed-meshheading:11697505-Core Binding Factor Alpha 2 Subunit,
pubmed-meshheading:11697505-DNA Probes,
pubmed-meshheading:11697505-Female,
pubmed-meshheading:11697505-Fluorescent Dyes,
pubmed-meshheading:11697505-Humans,
pubmed-meshheading:11697505-Leukemia, Myeloid,
pubmed-meshheading:11697505-Male,
pubmed-meshheading:11697505-Middle Aged,
pubmed-meshheading:11697505-Neoplasm, Residual,
pubmed-meshheading:11697505-Oncogene Proteins, Fusion,
pubmed-meshheading:11697505-RNA, Neoplasm,
pubmed-meshheading:11697505-Reference Standards,
pubmed-meshheading:11697505-Reproducibility of Results,
pubmed-meshheading:11697505-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11697505-Sensitivity and Specificity,
pubmed-meshheading:11697505-Transcription Factors,
pubmed-meshheading:11697505-Translocation, Genetic
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| pubmed:year |
2001
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| pubmed:articleTitle |
Quantitative detection of AML1-ETO rearrangement by real-time RT-PCR using fluorescently labeled probes.
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| pubmed:affiliation |
Department of Clinical Pathology, Hospital Universitario La Fe Valencia, Spain.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Evaluation Studies
|