Source:http://linkedlifedata.com/resource/pubmed/id/11693201
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
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| pubmed:dateCreated |
2001-11-5
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| pubmed:abstractText |
Three alpha1-AR subtypes have been cloned so far and are designated as alpha1a, alpha1b,, and alpha1d. Organ-specific distribution pattern and subtype-specific effects are known but not fully understood. To address a cell-type specific expression pattern in the heart we investigated expression pattern of alpha1-AR subtypes on RNA- and protein-level in heart tissue, cultured cardiomyocytes and non-myocytes of the rat. Each alpha1AR-subtype mRNA was present in neonatal and adult rat heart culture but the relative distribution pattern was significantly different. While the alpha1a-AR subtype is preferentially expressed in adult cardiomyocytes, the alpha1b-AR subtype was preferentially expressed in the non-myocyte cell fraction. The RT-PCR results were confirmed by Western-blotting (alpha1b) and immunocytochemical studies. Incubation with an alpha1-agonist (phenylephrine) for 72 h led to a significant reduction of the alpha1b-AR in neonatal heart cell culture on both mRNA and protein level. In contrast, incubation with an alpha1-antagonist (prazosin) induced a 1.6 fold upregulation of the alpha1a-AR mRNA without significant effects on radioligand binding and functional assay. The results indicate a distribution pattern of the alpha1-AR subtype which is specific for cell type and ontogeny of the rat heart and may be regulated by adrenergic agents.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Phenylephrine,
http://linkedlifedata.com/resource/pubmed/chemical/Prazosin,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Isoforms,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Adrenergic, alpha-1
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| pubmed:status |
MEDLINE
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| pubmed:month |
Aug
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| pubmed:issn |
0300-8177
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
224
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
69-79
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| pubmed:dateRevised |
2003-11-14
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| pubmed:meshHeading |
pubmed-meshheading:11693201-Animals,
pubmed-meshheading:11693201-Animals, Newborn,
pubmed-meshheading:11693201-Blotting, Western,
pubmed-meshheading:11693201-Cells, Cultured,
pubmed-meshheading:11693201-Dose-Response Relationship, Drug,
pubmed-meshheading:11693201-Gene Expression Regulation,
pubmed-meshheading:11693201-Heart,
pubmed-meshheading:11693201-Immunohistochemistry,
pubmed-meshheading:11693201-Male,
pubmed-meshheading:11693201-Myocardium,
pubmed-meshheading:11693201-Organ Specificity,
pubmed-meshheading:11693201-Phenylephrine,
pubmed-meshheading:11693201-Prazosin,
pubmed-meshheading:11693201-Protein Isoforms,
pubmed-meshheading:11693201-RNA, Messenger,
pubmed-meshheading:11693201-Rats,
pubmed-meshheading:11693201-Rats, Wistar,
pubmed-meshheading:11693201-Receptors, Adrenergic, alpha-1,
pubmed-meshheading:11693201-Reverse Transcriptase Polymerase Chain Reaction
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| pubmed:year |
2001
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| pubmed:articleTitle |
Expression of alpha1-adrenergic receptor subtypes in heart cell culture.
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| pubmed:affiliation |
Medical Clinic I, Department of Cardiology, Humboldt-University (Charité), Berlin, Germany.
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| pubmed:publicationType |
Journal Article
|