Source:http://linkedlifedata.com/resource/pubmed/id/11691641
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2001-11-5
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| pubmed:abstractText |
Previous studies from this laboratory have shown that salmon (S) calcitonin (CT)-like immunoreactive peptide (CTI) is synthesized and secreted by the anterior pituitary (AP) gland. These studies also co-localized CTI to gonadotropes, and demonstrated that SCT is a potent inhibitor of lactotrope function. However, the molecular structure of putative gonadotrope-derived CTI that inhibits lactotrope function has not been defined. The present studies cloned CT cDNA (pit-CT cDNA) from a mouse gonadotrope L beta T2 cell line using RT-PCR and rapid amplification of cDNA ends (RACE) techniques. Alignment of nucleotide sequences of pit-CT and mouse CT revealed greater than 99% homology between the sequences. The pit-CT cDNA was ligated into a mammalian expression vector, and the construct was transfected into L beta T2 cells. Two stable transfectant cell lines (CT.U6/A and B) were obtained by selection in G418. Subsequent S1-nuclease protection assay and immunocytochemistry results have shown that: (1) pit-CT peptide expressed by CT.U6 cell lines immunoreacted with GCT1-anti-SCT serum; (2) secretions of CT.U6 cells inhibited prolactin (PRL) release, PRL mRNA abundance and DNA synthesis of PRL-secreting GGH3 cells; and (3) CT.U6-induced inhibition was abolished by GCT1-anti-SCT serum. The studies also generated a riboprobe from the cloned pit-CT cDNA, and localized CT mRNA expression in gonadotropes of rat AP gland by in situ hybridization histochemistry. These results demonstrate that pit-CT mRNA is closely homologous to mouse CT mRNA; it is expressed by gonadotropes of the rat AP gland, and the peptide may significantly affect lactotrope function by inhibiting PRL release and cell proliferation.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcitonin,
http://linkedlifedata.com/resource/pubmed/chemical/Gonadotropins, Pituitary,
http://linkedlifedata.com/resource/pubmed/chemical/Luteinizing Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Prolactin,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
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| pubmed:status |
MEDLINE
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| pubmed:month |
Nov
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| pubmed:issn |
0022-0795
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
171
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
217-28
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:11691641-Animals,
pubmed-meshheading:11691641-Base Sequence,
pubmed-meshheading:11691641-Calcitonin,
pubmed-meshheading:11691641-Cell Line,
pubmed-meshheading:11691641-Cloning, Molecular,
pubmed-meshheading:11691641-Gene Expression,
pubmed-meshheading:11691641-Gonadotropins, Pituitary,
pubmed-meshheading:11691641-Immunohistochemistry,
pubmed-meshheading:11691641-In Situ Hybridization,
pubmed-meshheading:11691641-Luteinizing Hormone,
pubmed-meshheading:11691641-Mice,
pubmed-meshheading:11691641-Molecular Sequence Data,
pubmed-meshheading:11691641-Paracrine Communication,
pubmed-meshheading:11691641-Pituitary Gland, Anterior,
pubmed-meshheading:11691641-Polymerase Chain Reaction,
pubmed-meshheading:11691641-Prolactin,
pubmed-meshheading:11691641-Rats,
pubmed-meshheading:11691641-Recombinant Proteins,
pubmed-meshheading:11691641-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11691641-Sequence Alignment,
pubmed-meshheading:11691641-Transfection
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| pubmed:year |
2001
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| pubmed:articleTitle |
Calcitonin is expressed in gonadotropes of the anterior pituitary gland: its possible role in paracrine regulation of lactotrope function.
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| pubmed:affiliation |
Department of Pharmaceutical Sciences, Texas Tech University Health Sciences Center, 1300 Coulter, Amarillo, Texas 79106, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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