Source:http://linkedlifedata.com/resource/pubmed/id/11689246
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
2001-11-5
|
| pubmed:abstractText |
A method was developed for the large-scale bioconversion of novel 6-deoxyerythronolide B (6-dEB) analogs into erythromycin analogs. Erythromycin biosynthesis in Saccharopolyspora erythraea proceeds via the formation of a polyketide aglycone, 6-dEB, which is subsequently glycosylated, hydroxylated and methylated to yield the antibiotic erythromycin A. A modular polyketide synthase (PKS) directs 6-dEB synthesis using a dedicated set of active sites for the condensation of each of seven propionate units. Strategies based on genetic manipulation and precursor feeding are available for the efficient generation of novel 6-dEB analogs using a plasmid-based system in Streptomyces coelicolor. 6-dEB and 13-substituted 6-dEB analogs produced in this manner were fed to S. erythraea mutants which could not produce 6-dEB, yet retained their 6-dEB modification systems, and resulted in the generation of erythromycin A and 13-substituted erythromycin A analogs. Erythromycin B, C and D analogs were observed as intermediates of the process. Dissolved oxygen, temperature, the specific aglycone feed concentration, and pH were found to be important for obtaining a high yield of erythromycin A analogs. Cultivation conditions were identified which resulted in the efficient bioconversion of 6-dEB analogs into erythromycin A analogs, which this process demonstrated at the 100 l scale.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0168-1656
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| pubmed:author |
pubmed-author:AshleyGaryG,
pubmed-author:BurlingameMarkM,
pubmed-author:CadapanLawrenceL,
pubmed-author:CarneyJohnJ,
pubmed-author:CarrerasChristopherC,
pubmed-author:FrykmanScottS,
pubmed-author:LeafTimothyT,
pubmed-author:OuSallyS,
pubmed-author:PatelSajelS,
pubmed-author:VlasovaV VVV,
pubmed-author:WooElaineE,
pubmed-author:ZavalaStefanS
|
| pubmed:issnType |
Print
|
| pubmed:day |
18
|
| pubmed:volume |
92
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
217-28
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| pubmed:dateRevised |
2006-5-1
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| pubmed:meshHeading |
pubmed-meshheading:11689246-Bioreactors,
pubmed-meshheading:11689246-Biotechnology,
pubmed-meshheading:11689246-Biotransformation,
pubmed-meshheading:11689246-Culture Media,
pubmed-meshheading:11689246-Erythromycin,
pubmed-meshheading:11689246-Kinetics,
pubmed-meshheading:11689246-Molecular Structure,
pubmed-meshheading:11689246-Multienzyme Complexes,
pubmed-meshheading:11689246-Mutation,
pubmed-meshheading:11689246-Plasmids,
pubmed-meshheading:11689246-Saccharopolyspora,
pubmed-meshheading:11689246-Streptomyces
|
| pubmed:year |
2002
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| pubmed:articleTitle |
Saccharopolyspora erythraea-catalyzed bioconversion of 6-deoxyerythronolide B analogs for production of novel erythromycins.
|
| pubmed:affiliation |
Department of Process Science, Kosan Biosciences, Inc., 3832 Bay Center Place, Hayward, CA 94545, USA.
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| pubmed:publicationType |
Journal Article
|