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rdf:type |
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lifeskim:mentions |
umls-concept:C0002518,
umls-concept:C0007634,
umls-concept:C0019536,
umls-concept:C0020792,
umls-concept:C0021467,
umls-concept:C0021469,
umls-concept:C0086418,
umls-concept:C0547040,
umls-concept:C1514562,
umls-concept:C1880389,
umls-concept:C1883204,
umls-concept:C1883221
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pubmed:issue |
4
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pubmed:dateCreated |
2001-11-5
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pubmed:abstractText |
We found that human kinin-free high-molecular-weight kininogen (kf-HK) significantly inhibited vitronectin-mediated migration (haptotaxis) and invasive potentiation (haptoinvasion) of osteosarcoma (MG-63) cells but that HK, LK, the common heavy chain of HK and LK, and the light chain (D6(H)) of HK had no inhibitory effect. Recombinant GST-D5(H) (histidine-rich region of HK) obtained from Escherichia coli. (BL21) also inhibited both haptotaxis and haptoinvasion to about 30% of the control level in a dose-dependent manner. These findings suggest that a specific region of D5(H) is responsible for the inhibition of cell haptotaxis and haptoinvasion. Among the seven synthetic peptides covering D5(H), peptide H(479)KHGHGHGKHKNKGK(493) (P-5) inhibited both haptotaxis and haptoinvasion in a dose-dependent manner, suggesting that P-5 could possibly be utilized to prevent primary and secondary metastases of tumor cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2001 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
9
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pubmed:volume |
288
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
975-80
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:11689005-Amino Acid Sequence,
pubmed-meshheading:11689005-Cell Movement,
pubmed-meshheading:11689005-Collagen,
pubmed-meshheading:11689005-Dose-Response Relationship, Drug,
pubmed-meshheading:11689005-Drug Combinations,
pubmed-meshheading:11689005-Humans,
pubmed-meshheading:11689005-Kininogens,
pubmed-meshheading:11689005-Laminin,
pubmed-meshheading:11689005-Molecular Sequence Data,
pubmed-meshheading:11689005-Molecular Weight,
pubmed-meshheading:11689005-Neoplasm Invasiveness,
pubmed-meshheading:11689005-Neoplasm Metastasis,
pubmed-meshheading:11689005-Osteosarcoma,
pubmed-meshheading:11689005-Peptide Fragments,
pubmed-meshheading:11689005-Protein Structure, Tertiary,
pubmed-meshheading:11689005-Proteoglycans,
pubmed-meshheading:11689005-Recombinant Fusion Proteins,
pubmed-meshheading:11689005-Tumor Cells, Cultured,
pubmed-meshheading:11689005-Vitronectin
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pubmed:year |
2001
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pubmed:articleTitle |
Inhibition of vitronectin-mediated haptotaxis and haptoinvasion of MG-63 cells by domain 5 (D5(H)) of human high-molecular-weight kininogen and identification of a minimal amino acid sequence.
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pubmed:affiliation |
Department of Orthopedics, Nagoya City University Medical School, Mizuho-ku, Nagoya, 467-8601, Japan.
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pubmed:publicationType |
Journal Article
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