|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
4
|
|
pubmed:dateCreated |
2001-11-5
|
|
pubmed:abstractText |
The rat primary cultured-airway monolayer has been an excellent model for deciphering the ion channel after nystatin permeabilization of its basolateral or apical membrane. Inwardly rectifying K(+) currents were characterized across the basolateral membrane in symmetrical HCO(-)(3)-free high K(+) Ringer's solution (125 mM) in this study. The potency of K(+) channel inhibitors against K(+) conductance was Ba(2+) (IC(50) = 5 microM) > Cs(+) (IC(50) = 2 mM) >> glybenclamide (IC(50) > 5 mM) >> TEA (IC(50) >> 100 mM). The application of basolateral Cs(+) changed K(+) conductance into an oscillating current, and its frequency (holding voltage = -100 mV) increased with increase in concentration of basolateral Cs(+) (0.05-5 mM) and in degree of hyperpolarization. Addition of basolateral Cs(+) blocked inward current strongly at -100 mV and hardly at all at -60 mV, giving a sharp curvature to the I-V relation of the IRK current. RT-PCR, Western blotting, and immunohistochemical analyses showed that Kir2.1 might be present in basolateral membrane of tracheal epithelia and plasma membrane of pulmonary alveolar cells.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Nov
|
|
pubmed:issn |
0006-291X
|
|
pubmed:author |
|
|
pubmed:copyrightInfo |
Copyright 2001 Academic Press.
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
9
|
|
pubmed:volume |
288
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
914-20
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:11688996-Amino Acid Sequence,
pubmed-meshheading:11688996-Animals,
pubmed-meshheading:11688996-Blotting, Western,
pubmed-meshheading:11688996-Calcium,
pubmed-meshheading:11688996-Cell Membrane,
pubmed-meshheading:11688996-Cell Membrane Permeability,
pubmed-meshheading:11688996-Cell Polarity,
pubmed-meshheading:11688996-Cells, Cultured,
pubmed-meshheading:11688996-Cloning, Molecular,
pubmed-meshheading:11688996-Electric Conductivity,
pubmed-meshheading:11688996-Humans,
pubmed-meshheading:11688996-Immunohistochemistry,
pubmed-meshheading:11688996-Ion Channel Gating,
pubmed-meshheading:11688996-Membrane Potentials,
pubmed-meshheading:11688996-Molecular Sequence Data,
pubmed-meshheading:11688996-Myocardium,
pubmed-meshheading:11688996-Nasal Mucosa,
pubmed-meshheading:11688996-Potassium,
pubmed-meshheading:11688996-Potassium Channel Blockers,
pubmed-meshheading:11688996-Potassium Channels,
pubmed-meshheading:11688996-Potassium Channels, Inwardly Rectifying,
pubmed-meshheading:11688996-RNA, Messenger,
pubmed-meshheading:11688996-Rats,
pubmed-meshheading:11688996-Respiratory Mucosa,
pubmed-meshheading:11688996-Respiratory System,
pubmed-meshheading:11688996-Trachea
|
|
pubmed:year |
2001
|
|
pubmed:articleTitle |
Characterization of inwardly rectifying K(+) conductance across the basolateral membrane of rat tracheal epithelia.
|
|
pubmed:affiliation |
Department of Pathology, Dong-A Medical College, Busan, 602-103, South Korea.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
