Linked Life Data

11687893

Source:http://linkedlifedata.com/resource/pubmed/id/11687893

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2001-10-31
pubmed:abstractText
Malignant melanoma involving the oral cavity has a highly metastatic potential. Curative surgery is required to resect extensive oral tissues and often results in dysfunction as well as a severe cosmetic deformity in patients with the disease. An alternative technology for the local and sustained delivery of cytokines for cancer immunotherapy has been shown to induce tumor regression, suppression of metastasis, and development of systemic antitumor immunity. However, local immunization of the oral cavity has not previously been studied. In this study, we examined the efficacy of particle-mediated oral gene transfer on luciferase and green fluorescent protein production. The results showed that these proteins were more significantly expressed in oral mucosa than the skin, stomach, liver, and muscle. Using an established oral melanoma model in hamsters, particle-mediated oral gene gun therapy with interleukin (IL) 12 cDNA was then conducted. The results indicated that direct bombardment of mouse IL-12 cDNA suppressed tumor formation and improved the survival rate. The skin tumor model created by inoculation of melanoma cells was also significantly inhibited by the oral bombardment of IL-12 cDNA coupled with an irradiated melanoma vaccine administrated to the oral mucosa, compared to treatment with a percutaneous vaccine. IL-12 gene gun therapy, combined with an oral mucosal vaccine, induced interferon-gamma mRNA expression in the host spleen for a long time. These results suggest that immunization of oral mucosa may induce systemic antitumor immunity more efficiently than immunization of the skin and that oral mucosa may be one of the most suitable tissues for cancer gene therapy by means of particle-mediated gene transfer.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0929-1903
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
705-12
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11687893-Animals, pubmed-meshheading:11687893-Biolistics, pubmed-meshheading:11687893-Cancer Vaccines, pubmed-meshheading:11687893-Cricetinae, pubmed-meshheading:11687893-DNA, Complementary, pubmed-meshheading:11687893-DNA Primers, pubmed-meshheading:11687893-Gene Therapy, pubmed-meshheading:11687893-Green Fluorescent Proteins, pubmed-meshheading:11687893-Interferon-gamma, pubmed-meshheading:11687893-Interleukin-12, pubmed-meshheading:11687893-Luciferases, pubmed-meshheading:11687893-Luminescent Proteins, pubmed-meshheading:11687893-Male, pubmed-meshheading:11687893-Melanoma, Experimental, pubmed-meshheading:11687893-Mouth Mucosa, pubmed-meshheading:11687893-Mouth Neoplasms, pubmed-meshheading:11687893-Polymerase Chain Reaction, pubmed-meshheading:11687893-RNA, Messenger, pubmed-meshheading:11687893-Skin Neoplasms
pubmed:year
2001
pubmed:articleTitle
Gene gun-mediated oral mucosal transfer of interleukin 12 cDNA coupled with an irradiated melanoma vaccine in a hamster model: successful treatment of oral melanoma and distant skin lesion.
pubmed:affiliation
Division of Organ Replacement Research, Center for Molecular Medicine, Jichi Medical School, Tochigi 329-0498, Japan.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't