Source:http://linkedlifedata.com/resource/pubmed/id/11687880
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2001-11-5
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| pubmed:abstractText |
We tested the hypothesis that the primary cilium of renal epithelia is mechanically sensitive and serves as a flow sensor in MDCK cells using differential interference contrast and fluorescence microscopy. Bending the cilium, either by suction with a micropipette or by increasing the flow rate of perfusate, causes intracellular calcium to substantially increase as indicated by the fluorescent indicator, Fluo-4. This calcium signal is initiated by Ca2+-influx through mechanically sensitive channels that probably reside in the cilium or its base. The influx is followed by calcium release from IP3-sensitive stores. The calcium signal then spreads as a wave from the perturbed cell to its neighbors by diffusion of a second messenger through gap junctions. This spreading of the calcium wave points to flow sensing as a coordinated event within the tissue, rather than an isolated phenomenon in a single cell. Measurement of the membrane potential difference by microelectrode during perfusate flow reveals a profound hyperpolarization during the period of elevated intracellular calcium. We conclude that the primary cilium in MDCK cells is mechanically sensitive and responds to flow by greatly increasing intracellular calcium.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channel Blockers,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Heptanol,
http://linkedlifedata.com/resource/pubmed/chemical/Nitrendipine,
http://linkedlifedata.com/resource/pubmed/chemical/Verapamil
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| pubmed:status |
MEDLINE
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| pubmed:month |
Nov
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| pubmed:issn |
0022-2631
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
1
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| pubmed:volume |
184
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
71-9
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| pubmed:dateRevised |
2003-11-14
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| pubmed:meshHeading |
pubmed-meshheading:11687880-Animals,
pubmed-meshheading:11687880-Calcium,
pubmed-meshheading:11687880-Calcium Channel Blockers,
pubmed-meshheading:11687880-Calcium Signaling,
pubmed-meshheading:11687880-Cell Line,
pubmed-meshheading:11687880-Cell Polarity,
pubmed-meshheading:11687880-Cilia,
pubmed-meshheading:11687880-Dogs,
pubmed-meshheading:11687880-Enzyme Inhibitors,
pubmed-meshheading:11687880-Epithelial Cells,
pubmed-meshheading:11687880-Fluorescent Dyes,
pubmed-meshheading:11687880-Heptanol,
pubmed-meshheading:11687880-Kidney Tubules, Collecting,
pubmed-meshheading:11687880-Membrane Potentials,
pubmed-meshheading:11687880-Microscopy, Confocal,
pubmed-meshheading:11687880-Nitrendipine,
pubmed-meshheading:11687880-Stress, Mechanical,
pubmed-meshheading:11687880-Verapamil
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| pubmed:year |
2001
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| pubmed:articleTitle |
Bending the MDCK cell primary cilium increases intracellular calcium.
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| pubmed:affiliation |
NIH, NHLBI, LKEM, 10 Center Drive, Bldg. 10, Bethesda, MD 20892-1603, USA. praetorh@nhlbi.nih.gov
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| pubmed:publicationType |
Journal Article
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