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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
52
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pubmed:dateCreated |
2001-12-25
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pubmed:abstractText |
Interleukin-4 (IL-4) is a multifunctional cytokine that plays an important role in immune and inflammatory responses. Expression of the IL-4 gene is tightly controlled at the level of gene transcription by both positive and negative regulatory elements in the IL-4 promoter. Several constitutive nuclear factors have been identified that can interact with IL-4 promoter elements in DNA binding assays. Here we report that the zinc-finger protein YY-1 (Yin-Yang 1) can bind to multiple elements within the human IL-4 promoter. Cotransfection of Jurkat T cells with different IL-4 promoter/reporter constructs together with expression vectors encoding antisense, wild-type, or zinc finger-deleted mutant YY-1 suggested that YY-1 enhanced IL-4 promoter activity in a DNA-binding domain-dependent manner. Site-directed mutagenesis revealed that a proximal YY-1-binding site, termed Y0 ((-59)TCATTTT(-53)), was essential for YY-1-driven IL-4 promoter activity. In addition, cotransfected YY-1 enhanced both IL-4 promoter activity and endogenous IL-4 gene expression in nontransformed peripheral blood T cells. Thus, YY-1 positively regulates IL-4 gene expression in lymphocytes.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
28
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pubmed:volume |
276
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
48871-8
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:11687571-Calcimycin,
pubmed-meshheading:11687571-DNA-Binding Proteins,
pubmed-meshheading:11687571-Erythroid-Specific DNA-Binding Factors,
pubmed-meshheading:11687571-Gene Expression Regulation,
pubmed-meshheading:11687571-Genes, Reporter,
pubmed-meshheading:11687571-Humans,
pubmed-meshheading:11687571-Interleukin-4,
pubmed-meshheading:11687571-Ionophores,
pubmed-meshheading:11687571-Jurkat Cells,
pubmed-meshheading:11687571-Mutagenesis, Site-Directed,
pubmed-meshheading:11687571-Nuclear Proteins,
pubmed-meshheading:11687571-Promoter Regions, Genetic,
pubmed-meshheading:11687571-Protein Binding,
pubmed-meshheading:11687571-Transcription Factors,
pubmed-meshheading:11687571-Transcriptional Activation,
pubmed-meshheading:11687571-Transfection,
pubmed-meshheading:11687571-YY1 Transcription Factor,
pubmed-meshheading:11687571-Zinc Fingers
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pubmed:year |
2001
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pubmed:articleTitle |
Yin-Yang 1 activates interleukin-4 gene expression in T cells.
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pubmed:affiliation |
Division of Pulmonary and Critical Care Medicine, The Johns Hopkins Asthma and Allergy Center, Baltimore, Maryland 21224, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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