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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
21
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pubmed:dateCreated |
2001-10-30
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pubmed:databankReference |
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pubmed:abstractText |
The COP9 signalosome is a highly conserved eight-subunit protein complex initially defined as a repressor of photomorphogenic development in Arabidopsis. It has recently been suggested that the COP9 signalosome directly interacts and regulates SCF type E3 ligases, implying a key role in ubiquitin-proteasome mediated protein degradation. We report that Arabidopsis FUS11 gene encodes the subunit 3 of the COP9 signalosome (CSN3). The fus11 mutant is defective in the COP9 signalosome and accumulates significant amount of multi-ubiquitinated proteins. The same mutant is specifically impaired in the 26S proteasome-mediated degradation of HY5 but not PHYA, indicating a selective involvement in protein degradation. Reduction-of-function transgenic lines of CSN3 produced through gene co-suppression also accumulate multi-ubiquitinated proteins and exhibit diverse developmental defects. This result substantiates a hypothesis that the COP9 signalosome is involved in multifaceted developmental processes through regulating proteasome-mediated protein degradation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0950-1991
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
128
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4277-88
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:11684663-Amino Acid Sequence,
pubmed-meshheading:11684663-Arabidopsis,
pubmed-meshheading:11684663-Base Sequence,
pubmed-meshheading:11684663-Chromosome Mapping,
pubmed-meshheading:11684663-Cloning, Molecular,
pubmed-meshheading:11684663-Gene Expression Regulation, Plant,
pubmed-meshheading:11684663-Molecular Sequence Data,
pubmed-meshheading:11684663-Multiprotein Complexes,
pubmed-meshheading:11684663-Mutation,
pubmed-meshheading:11684663-Nuclear Proteins,
pubmed-meshheading:11684663-Peptide Hydrolases,
pubmed-meshheading:11684663-Plant Leaves,
pubmed-meshheading:11684663-Plant Proteins,
pubmed-meshheading:11684663-Plant Structures,
pubmed-meshheading:11684663-Protein Kinases,
pubmed-meshheading:11684663-Proteins,
pubmed-meshheading:11684663-Sequence Homology, Amino Acid,
pubmed-meshheading:11684663-Substrate Specificity,
pubmed-meshheading:11684663-Ubiquitin
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pubmed:year |
2001
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pubmed:articleTitle |
A role of Arabidopsis COP9 signalosome in multifaceted developmental processes revealed by the characterization of its subunit 3.
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pubmed:affiliation |
Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520-8104, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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