Linked Life Data

11684323

Source:http://linkedlifedata.com/resource/pubmed/id/11684323

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2001-10-30
pubmed:abstractText
CYP2A6 (cytochrome P450 2A6), which was first identified as the human coumarin 7-hydroxylase, is the most important enzyme in nicotine C-oxidation. The enzyme also metabolically activates the tobacco specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) in vitro. Polymorphisms in the CYP2A6 gene may thus impact on both smoking behavior and lung cancer susceptibility. Several different genotyping methods have been reported with conflicting results in the frequencies of CYP2A6 polymorphic variants. Thus we decided to perform a sequence analysis of the entire CYP2A6 gene. Sequencing confirmed the published CYP2A6 cDNA sequence. However, intron sequences differed considerably from the reported sequence of the CYP2A6*3 (v2) variant. Our analyses revealed that parts of introns shared homologies with the published sequence of CYP2A13. Based on our sequence data we developed a one step protocol for specific amplification of exon 3 of CYP2A6. The resulting PCR product can be used directly for restriction endonuclease digestion with XcmI and DdeI to determine the frequencies of the reported variant alleles CYP2A6*2 and CYP2A6*3. In a population of 305 African-Americans and 145 Caucasians, we found allele frequencies of 0.003 (2/610) for CYP2A6*2 and 0 (0/610) for CYP2A6*3 in African-Americans and allele frequencies of 0.014 (4/290) and 0 (0/290) in Caucasians. We conclude that both alleles are considerably less frequent in populations than previously reported.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0300-483X
pubmed:author
pubmed:issnType
Print
pubmed:day
30
pubmed:volume
168
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
259-68
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:11684323-African Continental Ancestry Group, pubmed-meshheading:11684323-Alleles, pubmed-meshheading:11684323-Aryl Hydrocarbon Hydroxylases, pubmed-meshheading:11684323-Cloning, Molecular, pubmed-meshheading:11684323-Cytochrome P-450 Enzyme System, pubmed-meshheading:11684323-DNA, pubmed-meshheading:11684323-European Continental Ancestry Group, pubmed-meshheading:11684323-Exons, pubmed-meshheading:11684323-Female, pubmed-meshheading:11684323-Gene Frequency, pubmed-meshheading:11684323-Genotype, pubmed-meshheading:11684323-Humans, pubmed-meshheading:11684323-Male, pubmed-meshheading:11684323-Mixed Function Oxygenases, pubmed-meshheading:11684323-Polymorphism, Genetic, pubmed-meshheading:11684323-Polymorphism, Restriction Fragment Length, pubmed-meshheading:11684323-Reverse Transcriptase Polymerase Chain Reaction
pubmed:year
2001
pubmed:articleTitle
Comparison of cytochrome P450 2A6 polymorphism frequencies in Caucasians and African-Americans using a new one-step PCR-RFLP genotyping method.
pubmed:affiliation
Analytisch-Biologisches Forschungslabor, Goethestr. 20, D-80336 München, Germany. frug-tp@bn-online.net
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't