Linked Life Data

11683393

Source:http://linkedlifedata.com/resource/pubmed/id/11683393

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 14
pubmed:dateCreated
2001-10-30
pubmed:abstractText
The integrin alphavbeta3 has been shown to be tightly linked to progression of human melanoma. In this study, using two clones from the K1735 murine melanoma system, we investigated the role of alphavbeta3 in metastasis. The highly metastatic K1735M2 cells express the alphavbeta3 integrin, whereas the poorly metastatic K1735C23 cells do not. When transduced with the beta3 integrin subunit cDNA, the K1735C23 cells produced lung lesions and, in two animals, cardiac metastases, whereas the parental C23 cells did not. By contrast, transduction of the full-length beta3 integrin antisense DNA into the K1735M2 cells suppressed metastatic colonization. To specifically investigate the activation of beta3 integrin-mediated pathways, the beta3-positive and the beta3-negative K1735 cells were plated onto vitronectin, a major matrix molecule of both primary and metastatic melanomas. Tyr397 of FAK was phosphorylated several times higher in beta3-expressing K1735 melanoma cells than in beta3-negative cells. To determine whether phosphorylation of FAK was associated with K1735 melanoma motility, we expressed the FAK-related non-kinase (FRNK) in the highly metastatic K1735M2 cells. Exogenous expression of FRNK suppressed phosphorylation of FAK at Tyr397 and decreased the invasive ability of these cells. In addition, expression of a constitutively active mutant Src in poorly metastatic K1735C23 cells increased invasion in vitro; whereas expression of a kinase-inactive Src mutant suppressed invasion. Our results suggest that signals initiated by alphavbeta3 promote metastasis in K1735 melanoma cells through the phosphorylation of FAK and activation of Src.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0021-9533
pubmed:author
pubmed:issnType
Print
pubmed:volume
114
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2665-72
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:11683393-Animals, pubmed-meshheading:11683393-Antigens, CD, pubmed-meshheading:11683393-Cell Movement, pubmed-meshheading:11683393-Focal Adhesion Kinase 1, pubmed-meshheading:11683393-Focal Adhesion Protein-Tyrosine Kinases, pubmed-meshheading:11683393-Integrin beta3, pubmed-meshheading:11683393-Melanoma, pubmed-meshheading:11683393-Mice, pubmed-meshheading:11683393-Mice, Inbred C3H, pubmed-meshheading:11683393-Neoplasm Transplantation, pubmed-meshheading:11683393-Phosphorylation, pubmed-meshheading:11683393-Platelet Membrane Glycoproteins, pubmed-meshheading:11683393-Protein-Tyrosine Kinases, pubmed-meshheading:11683393-Receptors, Vitronectin, pubmed-meshheading:11683393-Signal Transduction, pubmed-meshheading:11683393-Tumor Cells, Cultured, pubmed-meshheading:11683393-Tyrosine, pubmed-meshheading:11683393-src-Family Kinases
pubmed:year
2001
pubmed:articleTitle
Integrin alphavbeta3 mediates K1735 murine melanoma cell motility in vivo and in vitro.
pubmed:affiliation
Department of Stomatology, University of California, San Francisco 94143, USA.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't