Linked Life Data

11682502

Source:http://linkedlifedata.com/resource/pubmed/id/11682502

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2001-10-29
pubmed:abstractText
The utility of luciferase reporter mycobacteriophages (LRPs) for detection, identification, and antibiotic susceptibility testing of Mycobacterium tuberculosis was prospectively evaluated in a clinical microbiology laboratory in Mexico City, Mexico. Five hundred twenty-three consecutive sputum samples submitted to the laboratory during a 5-month period were included in this study. These specimens were cultivated in Middlebrook 7H9 (MADC), MGIT, and Löwenstein-Jensen (LJ) media. Of the 71 mycobacterial isolates recovered with any of the three media, 76% were detected with the LRPs, 97% were detected with the MGIT 960 method, and 90% were detected with LJ medium. When contaminated specimens were excluded from the analysis, the LRPs detected 92% (54 of 59) of the cultures. The median time to detection of bacteria was 7 days with both the LRPs and the MGIT 960 method. LRP detection of growth in the presence of p-nitro-alpha-acetylamino-beta-hydroxypropiophenone (NAP) was used for selective identification of M. tuberculosis complex (MTC) and compared to identification with BACTEC 460. Using the LRP NAP test, 47 (94%) out of 50 isolates were correctly identified as tuberculosis complex. The accuracy and speed of LRP antibiotic susceptibility testing with rifampin, streptomycin, isoniazid, and ethambutol were compared to those of the BACTEC 460 method, and discrepant results were checked by the conventional proportion method. In total, 50 MTC isolates were tested. The overall agreement between the LRP and BACTEC 460 results was 98.5%. The median LRP-based susceptibility turnaround time was 2 days (range, 2 to 4 days) compared to 10.5 days (range, 7 to 16 days) by the BACTEC 460 method. Phage resistance was not detected in any of the 243 MTC isolates tested. Mycobacteriophage-based approaches to tuberculosis diagnostics can be implemented in clinical laboratories with sensitivity, specificity, and rapidity that compare favorably with those of the MGIT 960 and BACTEC 460 methods. The phages currently provide the fastest phenotypic assay for susceptibility testing.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11682502-10074539, http://linkedlifedata.com/resource/pubmed/commentcorrection/11682502-10423219, http://linkedlifedata.com/resource/pubmed/commentcorrection/11682502-10794937, http://linkedlifedata.com/resource/pubmed/commentcorrection/11682502-10815117, http://linkedlifedata.com/resource/pubmed/commentcorrection/11682502-13927224, http://linkedlifedata.com/resource/pubmed/commentcorrection/11682502-6429191, http://linkedlifedata.com/resource/pubmed/commentcorrection/11682502-6470677, http://linkedlifedata.com/resource/pubmed/commentcorrection/11682502-8337467, http://linkedlifedata.com/resource/pubmed/commentcorrection/11682502-8484123, http://linkedlifedata.com/resource/pubmed/commentcorrection/11682502-8735121, http://linkedlifedata.com/resource/pubmed/commentcorrection/11682502-9399524, http://linkedlifedata.com/resource/pubmed/commentcorrection/11682502-9614254, http://linkedlifedata.com/resource/pubmed/commentcorrection/11682502-9854062, http://linkedlifedata.com/resource/pubmed/commentcorrection/11682502-9986844
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
39
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3883-8
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Luciferase reporter mycobacteriophages for detection, identification, and antibiotic susceptibility testing of Mycobacterium tuberculosis in Mexico.
pubmed:affiliation
Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, California 94143-0134, USA. niaz@itsa.ucsf.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't