Linked Life Data

11682223

Source:http://linkedlifedata.com/resource/pubmed/id/11682223

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2001-10-29
pubmed:abstractText
The separation of the lipophilic compounds in extracts of Hypericum perforatum L. is demonstrated in a non-aqueous capillary electrophoresis system with reversed electro-osmotic flow. Solvent mixtures of methanol, dimethylsulfoxide and N-methylformamide were used for the electrophoresis media, with addition of ammonium acetate and sodium acetate as electrolytes. The flow was reversed by the addition of the polycation hexadimethrine bromide, and thus negative voltage was applied. The method shows baseline separation between the four hypericins-protopseudohypericin, pseudohypericin, protohypericin and hypericin-whereas total baseline separation between the two hyperforins-hyperforin and adhyperforin-was not achieved. Using a fused-silica capillary (30 cm x 25 microm ID) and a voltage of -25 kV the analysis time of the hypericins and hyperforins was obtainable within 3 min. Application of the method with a fused-silica capillary of a larger internal diameter (48.5 cm x 50 microm ID) and a voltage of -20 kV resulted in analysis times of 8 min, but also lower limits of detection. The maximal attainable voltage was applied in both cases. Simultaneous separation of the flavonoids-although less efficient-may also be achieved. The technique of non-aqueous capillary electrophoresis with reversed electro-osmotic flow provides a very fast technique to evaluate the composition of hypericins and hyperforins in extracts of Hypericum perforatum L.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0731-7085
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
167-76
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Separation of hypericins and hyperforins in extracts of Hypericum perforatum L. using non-aqueous capillary electrophoresis with reversed electro-osmotic flow.
pubmed:affiliation
Department of Analytical and Pharmaceutical Chemistry, The Royal Danish School of Pharmacy, Universitetsparken 2, DK-2100, Copenhagen, Denmark.
pubmed:publicationType
Journal Article